Strain Data Sheet

RBRC09860

Strain Information

Image
BRC No.RBRC09860
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(Thy1-FUS<*>deltaNLS)1Dit
Former Common namedeltaNLF-FUStg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentA human FUS cDNA without a nuclear localization signal was inserted into the pTSC21k vector, which encodes the 8.1 kb murine Thy1.2 expression cassette. This construct was Not I digested to remove vector sequences, co-injected into the pronucleus of fertilized eggs from Slc:BDF1 strain mice. Founders were bred and backcrossed with C57Bl6/J mice to establish transgenic lines.
Strain descriptionA transgenic (tg) mouse overexpressing exogenous FUS without a nuclear localization signal (ΔNLS-FUS) develop progressive spastic motor deficits and neuronal loss in the motor cortex. The ΔNLS-FUS protein was restricted to the cytoplasm and formed ubiquitin/p62-positive aggregates. Endogenous FUS expression, nuclear localization, and splicing activity were not altered, indicating that mislocated FUS is sufficient for proteinopathy. RNA-sequence analysis revealed specific transcriptome alterations, including genes regulating dynein-associated molecules and endoplasmic reticulum stress.
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6JJcl]
ReferencesBrain. 2016 Sep;139(Pt 9):2380-94. doi: 10.1093/brain/aww161. Epub 2016 Jun 30. 27368346

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FUSfused in sarcomaUNFUS

Ordering Information

供与核酸マウス　Thy-1 promoterとマウスThy-1のexon3,4, ヒト human FUS cDNA without a nuclear localization signalを挿入, マウス Thy-1exon3,4
Research application
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Brain 2016 Sep;139(Pt 9):2380-94. doi: 10.1093/brain/aww161. Epub 2016 Jun 30.
Depositor伊東　大介（慶應義塾大学）
Strain Status凍結胚のアイコン凍結胚
Strain Availability受注後、検査を施して提供
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data