Strain Information | |
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Image | |
BRC No. | RBRC09856 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | B6D2-Gk2<em1Osb> |
Former Common name | Gk2<-7/wt> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Hirotaka Kato and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2014. BDF1 background. |
Strain description | Gk2 mutant mice generated by the CRISPR/Cas9 technique. 7 bp deletion.Gk2<em1Osb>, [Deleted sequence]:tgactggctgttgtgtctcctaactggcaTCACCAGGGAAACAGTCCACTGACCAGTTTCATGGCAGCCTCGAAGCAAACCTCTGCGGGACCGTTGGTAGGAGCGGTGG[TCCAGGG]AACCAACTCCACTCGCTTTCTAGTTTTCAATAGCAAAACAGCAGAACTAGTTTGCTCTCATCAGGTGGAACTGACACAAGAATACCCAAAAGAAGGATGGGTGGAGCAAGACCCCAAGGAAATCCTTAAATCTGTCTATGAGTGCATAGCGAAAGCTTGTGAAAAACTTGCTGAAGTGAACATTGATATTTCCAACATTAAAGCTATCGGCGTGAGTAACCAGCGGGAAACCACTGTGGTCTGGGACAAGTTCACGGGAGACCCTCTGTACAATGCCGTGGTGTGGCTTGATCTCAGAACCCAGTCTACTGTGGAGACTCTCACTAAGAAAATCCCAGGAAATAGCAACTTTGTCAAGTCTAAGACCGGCCTTCCTCTTAGCACTTACTTCAGTGCTGTGAAGCTGCGGTGGATGCTGGACAACCTAAGACCCATTCAAAAGGCTGTTGAGGAAGGCAGAGCCATGTTTGGAACCATCGAC |
Colony maintenance | |
References | J. Reprod. Dev., 65(2):155-162 (2019). 30662012 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gk2glycerol kinase 25Gk2<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University |
Ordering Information | |
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Donor DNA | human hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Mouse a part of Gk2gene |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Reprod. Dev. 65: 155-162 (2019).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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No Data |