Strain Data Sheet

RBRC09851

Strain Information

Image
BRC No.RBRC09851
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Ssmem1<em1Osb>
Former Common nameSsmem1<-5/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Zhang Qian and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2015. BDF1 background.
Strain descriptionSsmem1 mutant mice generated by the CRISPR/Cas9 technique. 5 bp deletion.Ssmem1<em1Osb>, [Deleted sequence]:gcactcatttaacagggctgactgtttaaagttagaccttagggaagggccttgggccatggcttaatatgatggtttaatagatgctactcagtgcagctgacccagtccatcttactgtatctctgaaacagATGTCTGA[GAGCA]AACAGGACGAAGACAGCGGGACCAGTGCGTCACTCAGTAAAGgtgagagtcgggggtgcttggagtcgggctctgagagagggttgtgtcactgagtgtggagaacggatagcacaagtgaaaagcagagaggttccaaggcctcatcacgccagcaaagacc
Colony maintenance
References
Knockout of serine-rich single-pass membrane protein 1 (Ssmem1) causes globozoospermia and sterility in male micedagger.
Nozawa K, Zhang Q, Miyata H, Devlin D J, Yu Z, Oura S, Koyano T, Matsuyama M, Ikawa M, Matzuk M M
Biol. Reprod., 103(2):244-253 (2020). 32301969

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ssmem1
MGI:1922897		serine-rich single-pass membrane protein 16Ssmem1<em1Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal gametes(MP:0001124)

  • male infertility(MP:0001925)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistance gene, Mouse a part of Ssmem1gene
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod., 103(2):244-253 (2020). doi: 10.1093/biolre/ioaa040.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data