Strain Information | |
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Image | |
BRC No. | RBRC09843 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | STOCK Del(9Gm17677-Gm17689)1Osb |
Former Common name | PateN-G<-841 kb/wt> |
H-2 Haplotype | |
ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
Background strain | |
Appearance | |
Strain development | Developed by Taichi Noda and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2015. EGR-G01 ES cell was used to generate chimera mice. Mice were further crossed with B6D2F1. Mixed genetic background. |
Strain description | Mutant mice generated by the CRISPR/Cas9 technique. 841 kb deletion at chromosome 9.Del(9Gm17677-Gm17689)1Osb, [Deleted sequence]:AGGAGTACTGATTTTGTACAAGT[ctcattcaatgggtgagtgtaat (about 841 kb deletion) caggagtatcaagaggtattttccaattgcagt]GGGATCCTAGGAG |
Colony maintenance | |
References | Proc. Natl. Acad. Sci. USA, 116(37):18498-18506 (2019). doi:10.1073/pnas.1908736116. 31455729 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter |
Ordering Information | |
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Donor DNA | human hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Mouse a part of PateN-PateG gene, jellyfish GFP cDNA |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18498-18506. doi: 10.1073/pnas.1908736116.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |