Strain Data Sheet

RBRC09826

Strain Information

Image
BRC No.RBRC09826
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Fndc8<em1Osb>
Former Common nameFndc8<-1/-1>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2014. B6D2 genetic background.
Strain descriptionFndc8 mutant mice generated by the CRISPR/Cas9 technique. 1 bp deletion at Exon 1.Fndc8<em1Osb>, [Deleted sequence]gattgtgaagtcagagcactgcaaaccttctcacccaaatgaagggtgagacctttgcctcAGTTTTTCTCTGCTTCCTGCCAGCTGGTTGTCCTGGAGGGTGGTGGCAACCCTGCGCCCCACCCACACCAGATGGCGACGGTATTCTGTAAA[G]TGGGGGGTGGAGAGGAGGCTGTACCGAAGAAGGAAGCCCTCAATGTTATCAATGTGATTGACCAACTGCCAAAGCCCTGTCCAAATCCTAAGTTTATTAACCGGTCCATGGCTACCAAAGGCCTCCTGCTCCCGTCAAGACGCAGCTTGGCCAGCTTCTCAGAGGAAGAAAACACCGATGTCATgtaagacacgatttggggtcctcatttctaaggctggagaagctttgcagcccactgtggtgggtccttcagggaagaacatatcaggaggttccccatgcacccgccctgagccccacatcagttttgagagg
Colony maintenance
References
CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in micedagger.
Lu Y, Oura S, Matsumura T, Oji A, Sakurai N, Fujihara Y, Shimada K, Miyata H, Tobita T, Noda T, Castaneda J M, Kiyozumi D, Zhang Q, Larasati T, Young S A M, Kodani M, Huddleston C A, Robertson M J, Coarfa C, Isotani A, Aitken R J, Okabe M, Matzuk M M, Garcia T X, Ikawa M
Biol. Reprod., 101(2):501-511 (2019). 31201419

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fndc8
MGI:1926169		fibronectin type III domain containing 811Fndc8<em1Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • reproductive system phenotype(MP:0005389)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistance gene, Mouse a part of Fndc8 gene
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod., 101(2):501-511 (2019).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data