Strain Data Sheet

RBRC09820

Strain Information

Image
BRC No.RBRC09820
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129;B6-Prdm13<tm1.2Tfur>
Former Common namePrdm13-KO
H-2 Haplotype
ES Cell lineJM8A3 [C57BL/6N-A<tm1Brd>]
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa (2014), Institute for Protein Research, Osaka University. JM8A3 ES(C57BL/6N)cells were used. This strain was generated by crossing a strain carrying floxed Prdm13 (RBRC09371) with CAG-Cre line.
Strain descriptionPrdm13(PR domain containing 13)knockout mice. Exon 2 and 3 were deleted.
Colony maintenance
References
Prdm13 regulates subtype specification of retinal amacrine interneurons and modulates visual sensitivity.
Watanabe S, Sanuki R, Sugita Y, Imai W, Yamazaki R, Kozuka T, Ohsuga M, Furukawa T
J. Neurosci., 35, 8004-8020 (2015). 25995483

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Prdm13PR domain containing 134Prdm13targeted mutation 1.2, Takahisa Furukawa
loxPphage P1 loxP4loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal optokinetic reflex(MP:0009619)

  • abnormal retina inner plexiform layer morphology(MP:0003734)

  • abnormal visual contrast sensitivity(MP:0011831)

  • decreased amacrine cell number(MP:0008106)

  • thin retina inner nuclear layer(MP:0008511)
    • more 1 phenotypes
  • thin retina inner plexiform layer(MP:0008513)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP site, mouse Prdm13 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 35, 8004-8020 (2015).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
    DepositorTakahisa Furukawa (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data