Strain Data Sheet
RBRC09806
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC09806 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6.Cg-Tg(CAG-cat,-EGFP)39Miya |
| Former Common name | CAG-CAT-EGFP mice, CAG-LSL-EGFP mice |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6JJcl |
| Appearance | |
| Strain development | Developed by Jun-ich Miyazaki in 1999. Transgene was injected into BDF1, and then mice were backcrossed to C57BL/6 for more than 30 generations. |
| Strain description | Transgenic reporter mice for Cre recombinase. Floxed cat gene can be excised by Cre recombinase, resulting in EGFP expression. |
| Colony maintenance | |
| References | A novel reporter mouse strain that expresses enhanced green fluorescent protein upon Cre-mediated recombination. S Kawamoto, H Niwa, F Tashiro, S Sano, G Kondoh, J Takeda, K Tabayashi, J Miyazaki FEBS Lett., 470, 263-268 (2000). 10745079 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2026/03/16Room:3-5Rack:DSentinel mouse program 2026/01/13Room:3-5Rack:DSentinel mouse program 2025/11/04Room:3-5Rack:DSentinel mouse program 2025/08/18Room:3-5Rack:DSentinel mouse program 2025/06/09Room:3-5Rack:DSentinel mouse program 2025/03/10Room:3-5Rack:DSentinel mouse program 2024/12/09Room:3-5Rack:DSentinel mouse program 2024/09/09Room:3-5Rack:DSentinel mouse program 2024/06/10Room:3-5Rack:DSentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | UN | EGFP | ||||
| cat | E. coli chloramphenicol acetyltransferase (CAT) | UN | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | ||||
| loxP | phage P1 loxP | UN | loxP | ||||
| loxP | phage P1 loxP | UN | loxP | ||||
Phenotype | |
|---|---|
| Phenotype annotation from literatures by Mammalian phenotype ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, E. coli Chloramphenicol acetyltransferase gene, jellyfish Enhanced green fluorescent protein cDNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Letters, 470, 263-268 (2000). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for- profit research is requested to enter into a Material Transfer Agreement with Osaka University. |
| Depositor | Jun-ichi Miyazaki (Osaka University) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
|---|
Nagao M, Maekawa T, Yamazaki A, Ogata T. Chromatin remodeler Chd7 regulates reactive astrogliosis after ischemic stroke. Cell Rep 44(12) 116590(2025) 41264417 |
Baba Y, Onishi-Sakamoto S, Ide K, Nishifuji K. Nestin is a marker of unipotent embryonic and adult progenitors differentiating into an epithelial cell lineage of the hair follicles. Sci Rep 12(1) 17820(2022) 36280775 |
Hosoyama T. Possible application of muscle specific conditional mouse-derived induced pluripotent stem cells for muscle research. Biochem Biophys Rep 21 100744(2020) 32025579 |