Strain Data Sheet

RBRC09798

Strain Information

Image
BRC No.RBRC09798
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ano5<tm1.2Naga>
Former Common nameTMEM16E KO
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shigekazu Ngata, Jun Suzuki and Sayuri Gyobu at Osaka University in 2012. Bruce 4 ES cell line was used. Exon 2 and Neomycin resistance gene were removed by crossing CAG-Cre mice.
Strain descriptionAno5(anoctamin 5, Tmem16e) knockout mice.
Colony maintenance
References
Next generation sequencing on patients with LGMD and nonspecific myopathies: Findings associated with ANO5 mutations.
Savarese M, Di Fruscio G, Tasca G, Ruggiero L, Janssens S, De Bleecker J, Delpech M, Musumeci O, Toscano A, Angelini C, Sacconi S, Santoro L, Ricci E, Claes K, Politano L, Nigro V
Neuromuscul. Disord., 25, 533-541 (2015). 25891276

A Role of TMEM16E Carrying a Scrambling Domain in Sperm Motility.
Gyobu S, Miyata H, Ikawa M, Yamazaki D, Takeshima H, Suzuki J, Nagata S
Mol. Cell Biol., 36, 645-659 (2015). 26667038

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ano5anoctamin 57Ano5targeted mutation 1.2, Shigekazu Nagata
  • autosomal recessive limb-girdle muscular dystrophy type 2L(DisGeNET, MedGEN)

  • gnathodiaphyseal dysplasia(DisGeNET, MedGEN)

  • Miyoshi muscular dystrophy 3(MedGEN)
  • loxPphage P1 loxP7loxP
  • autosomal recessive limb-girdle muscular dystrophy type 2L(DisGeNET, MedGEN)

  • gnathodiaphyseal dysplasia(DisGeNET, MedGEN)

  • Miyoshi muscular dystrophy 3(MedGEN)
  • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • behavior/neurological phenotype(MP:0005386)

  • decreased activated sperm motility(MP:0009280)

  • decreased sperm progressive motility(MP:0020451)

  • homeostasis/metabolism phenotype(MP:0005376)

  • muscle phenotype(MP:0005369)
  • more 2 phenotypes
  • reduced male fertility(MP:0001922)

  • reproductive system phenotype(MP:0005389)
  • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, mouse Ano6 genome DNA
    Research applicationCre/loxP system
    Mouse Models for Human Disease
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 36, 645-659 (2015). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorShigekazu Nagata (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Li X, Wang L, Wang H, Qin A, Qin X.
    Ano5 modulates calcium signaling during bone homeostasis in gnathodiaphyseal dysplasia.
    NPJ Genom Med 7(1) 48(2022) 35982081