Strain Information | |
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Image | |
BRC No. | RBRC09798 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Ano5<tm1.2Naga> |
Former Common name | TMEM16E KO |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Shigekazu Ngata, Jun Suzuki and Sayuri Gyobu at Osaka University in 2012. Bruce 4 ES cell line was used. Exon 2 and Neomycin resistance gene were removed by crossing CAG-Cre mice. |
Strain description | Ano5(anoctamin 5, Tmem16e) knockout mice. |
Colony maintenance | |
References | Next generation sequencing on patients with LGMD and nonspecific myopathies: Findings associated with ANO5 mutations. Savarese M, Di Fruscio G, Tasca G, Ruggiero L, Janssens S, De Bleecker J, Delpech M, Musumeci O, Toscano A, Angelini C, Sacconi S, Santoro L, Ricci E, Claes K, Politano L, Nigro V Neuromuscul. Disord., 25, 533-541 (2015). 25891276A Role of TMEM16E Carrying a Scrambling Domain in Sperm Motility. Gyobu S, Miyata H, Ikawa M, Yamazaki D, Takeshima H, Suzuki J, Nagata S Mol. Cell Biol., 36, 645-659 (2015). 26667038 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Ano5 | anoctamin 5 | 7 | Ano5 | targeted mutation 1.2, Shigekazu Nagata | |||
loxP | phage P1 loxP | 7 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Phage P1 loxP site, mouse Ano6 genome DNA |
Research application | Cre/loxP system Mouse Models for Human Disease |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 36, 645-659 (2015). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Shigekazu Nagata (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Li X, Wang L, Wang H, Qin A, Qin X. Ano5 modulates calcium signaling during bone homeostasis in gnathodiaphyseal dysplasia. NPJ Genom Med 7(1) 48(2022) 35982081 |