Strain Data Sheet

RBRC09796

Strain Information

Image
BRC No.RBRC09796
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ano6<tm1.1Naga>
Former Common nameTMEM16F Flox
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shigekazu Ngata, Jun Suzuki and Toshihiro Fujii at Osaka University in 2013. Bruce 4 ES cell line was used. Neomycin resistance gene was removed by crossing CAG-FLPe mice. A pair of loxP are flanking exon 2.
Strain descriptionFloxed mice for Ano6(anoctamin 6, Tmem16f).
Colony maintenance
References
Calcium-dependent phospholipid scrambling by TMEM16F.
Jun Suzuki, Masato Umeda, Peter J Sims, Shigekazu Nagata
Ｎature, 468, 834-838 (2010). 21107324

Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members.
Jun Suzuki, Toshihiro Fujii, Takeshi Imao, Kenji Ishihara, Hiroshi Kuba, Shigekazu Nagata
J. Biol. Chem., 288, 13305-13316 (2013). 23532839

TMEM16F is required for phosphatidylserine exposure and microparticle release in activated mouse platelets.
Toshihiro Fujii, Asuka Sakata, Satoshi Nishimura, Koji Eto, Shigekazu Nagata
Proc. Natl. Acad. Sci. USA, 112, 12800-12805 (2015). 26417084

Health Report

Examination Date / Room / Rack2025/07/28Room:4-ARack:ESentinel mouse program
2025/05/19Room:4-ARack:ESentinel mouse program
2025/02/17Room:4-ARack:ESentinel mouse program
2024/11/18Room:4-ARack:ESentinel mouse program
2024/08/19Room:4-ARack:ESentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ano6
MGI:2145890		anoctamin 615Ano6<tm1.1Naga>
MGI:5538269		targeted mutation 1.1, Shigekazu Nagata
  • Scott syndrome(MedGEN)
    • Frt yeast FRT (flippase recombination target) site15Frt
    loxP phage P1 loxP15loxP
    loxP phage P1 loxP15loxP

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Ano6 genome DNA
    Research applicationCre/loxP system
    FLP/frt system
    Mouse Models for Human Disease
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 112, 12800-12805 (2015). J. Biol. Chem., 288, 13305-13316 (2013). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorShigekazu Nagata (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Sakata A, Tatsumi K, Matsumoto N, Mackman N, Harada S, Kawasaki R, Okuyama-Nishida Y, Soeda T, Nogami K, Shima M.
    Extravascular coagulation regulates haemostasis independently of activated platelet surfaces in an in vivo mouse model.
    Commun Biol 8(1) 390(2025) 40055470