Strain Information | |
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Image | |
BRC No. | RBRC09796 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Ano6<tm1.1Naga> |
Former Common name | TMEM16F Flox |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Shigekazu Ngata, Jun Suzuki and Toshihiro Fujii at Osaka University in 2013. Bruce 4 ES cell line was used. Neomycin resistance gene was removed by crossing CAG-FLPe mice. A pair of loxP are flanking exon 2. |
Strain description | Floxed mice for Ano6(anoctamin 6, Tmem16f). |
Colony maintenance | |
References | Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members. Suzuki J, Fujii T, Imao T, Ishihara K, Kuba H, Nagata S J. Biol. Chem., 288, 13305-13316 (2013). 23532839Calcium-dependent phospholipid scrambling by TMEM16F. Suzuki J, Umeda M, Sims P J, Nagata S Nature, 468, 834-838 (2010). 21107324TMEM16F is required for phosphatidylserine exposure and microparticle release in activated mouse platelets. Fujii T, Sakata A, Nishimura S, Eto K, Nagata S Proc. Natl. Acad. Sci. USA, 112, 12800-12805 (2015). 26417084 |
Health Report | |
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Examination Date / Room / Rack | 2024/08/19Room:4-ARack:E 2024/05/20Room:4-ARack:E 2024/02/19Room:4-ARack:E 2023/11/20Room:4-ARack:E 2023/08/21Room:4-ARack:E 2023/05/22Room:4-ARack:E 2023/02/20Room:4-ARack:E 2023/02/20Room:4-ARack:E |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Ano6 | anoctamin 6 | 15 | Ano6 | targeted mutation 1.1, Shigekazu Nagata | |||
Frt | yeast FRT (flippase recombination target) site | 15 | Frt | ||||
loxP | phage P1 loxP | 15 | loxP | ||||
loxP | phage P1 loxP | 15 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Ano6 genome DNA |
Research application | Cre/loxP system FLP/frt system Mouse Models for Human Disease |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 112, 12800-12805 (2015). J. Biol. Chem., 288, 13305-13316 (2013). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Shigekazu Nagata (Osaka University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |