Strain Data Sheet

RBRC09782

Strain Information

Image
BRC No.RBRC09782
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Drc7<tm1a(KOMP)Osb>/32
Former Common nameCcdc135#32, Drc7 KO
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Miyata and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2014. C57BL/6N genetic background. EGR-G101 ES cell was used.
Strain descriptionFloxe mice for Drc7 (dynein regulatory complex subunit 7, Ccdc135). A pair of loxP flanks exon 9, 10 and 11.
Colony maintenance
References
Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice.
Morohoshi A, Miyata H, Shimada K, Nozawa K, Matsumura T, Yanase R, Shiba K, Inaba K, Ikawa M
PLoS Genet., 16(1):e1008585 (2020). 31961863

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Drc7
MGI:2685616		dynein regulatory complex subunit 78Drc7<tm1a(KOMP)Osb>
MGI:4451680		targeted mutation 1a, Research Institute for Microbial Diseases, Osaka University
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)8En2
Frt yeast FRT (flippase recombination target) site8Frt
Frt yeast FRT (flippase recombination target) site8Frt
Ires internal ribosomal entry site (EMCV)8
lacZ beta-galactosidase (E. coli)8
loxP phage P1 loxP8loxP
loxP phage P1 loxP8loxP
loxP phage P1 loxP8loxP
neo neomycin resistance gene (E. coli)8
poly A polyadenylic acid additional signal8poly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal sperm axoneme morphology(MP:0009838)

  • abnormal sperm fibrous sheath morphology(MP:0030590)

  • abnormal sperm head morphology(MP:0009230)

  • abnormal sperm mitochondrial sheath morphology(MP:0009832)

  • abnormal sperm motility(MP:0002674)
    • more 3 phenotypes
  • male infertility(MP:0001925)

  • short sperm flagellum(MP:0009239)

  • small testis(MP:0001147)
    • Detailed phenotype data

    Ordering Information

    Donor DNAchicken beta-actin promoter, rabbit beta-globin polyadenylation signal, CMV CMV IE enhancer, mouse proacrosin signal peptide, mouse acrosin N-ternimal peptide, jellyfish GFP cDNA, mouse acrosin promoter, mouse Drc7 genomic DNA, mouse En2 splice acceptor (En2 intron2/exon 3 splice acceptor), EMCV Internal ribosomal entry site (IRES), E.coli. lacZ, SV40 polyadenylation site, human Beta-actin promoter, E.coli. Neomysin phosphotransferase gene (neoR) , Yeast FRT site, Phage P1 loxP site
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 16(1):e1008585 (2020).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data