Strain Data Sheet

RBRC09769

Strain Information

Image
BRC No.RBRC09769
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm2.1(CAG-HIST1H2BJ)Shna>/24
Former Common nameAll-flagH2B
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Shinichi Nakagawa, RIKEN. C57BL/6 background. TT2 ES cell line [(C57BL/6NCrlj x CBA/JNCrlj)F1] was used. Mixed genetic background.
Strain descriptionKnock-in mice for overexpression of human histone cluster 1, H2bj (HIST1H2BJ). RBRC09768 is a strain carrying targeted insertion of CAG-loxP-pgk-neo-3xpA-loxP-Flag-HIST1H2BJ-pA cassette into the mouse Rosa26 locus. Flag-tag was fused to HIST1H2BJ N-terminally. This strain was generated by further crossing FVB-Tg(Ddx4-cre)1Dcas/J to delete pgk-neo-3xpA.
Colony maintenance
ReferencesSci. Rep., 8(1):1143 (2018). 29348483

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm2.1(CAG-HIST1H2BJ)Shna>targeted mutation 2.1, Shinichi Nakagawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)6FLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)6GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HIST1H2BJhistone cluster 1, H2bj (human)6HIST1H2BJCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Ordering Information

供与核酸CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Enterobacteria phage P1 loxP site, FLAG tag sequence (synthetic), human histone cluster 1, H2bj (HIST1H2BJ), bovine growth hormone polyA signal, mouse ROSA26 genomic DNA
Research applicationCre/loxP system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Sci Rep., 8(1):1143 (2018).
研究成果の公表にあたって謝辞の表明を必要とする。
Depositor中川　真一（国立研究開発法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data