Strain Information | |
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Image | |
BRC No. | RBRC09766 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Gt(ROSA)26Sor<tm1(CAG-Hnrnpu)Shna>/15 |
Former Common name | flag-hnRNPU(F5)#15 |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Shinichi Nakagawa, RIKEN. C57BL/6 background. TT2 ES cell line [(C57BL/6NCrlj x CBA/JNCrlj)F1] was used. Mice were backcrossed to C57BL/6 for 5 generations. CDB1192K. |
Strain description | Knock-in mice for Cre-dependent overexpression of mouse heterogeneous nuclear ribonucleoprotein U (Hnrnpu). Targeted insertion of CAG-loxP-pgk-neo-3xpA-loxP-Flag-Hnrnpu-pA cassette into the mouse Rosa26 locus. Flag-tag was fused to Hnrnpu N-terminally. A strain deleting pgk-neo-3xpA by Cre is available as RBRC09767. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-Hnrnpu)Shna>targeted mutation 1, Shinichi Nakagawa Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)6FLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)6GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hnrnpuheterogeneous nuclear ribonucleoprotein U6HnrnpuCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA6 |
Ordering Information | |
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供与核酸 | マウス(Mus musculus) ROSA26, サイトメガロウィルス(cytomegalovirus) cytomegalovirus enhancer, ニワトリ(Gallus gallus) β-actin promoter, ウサギ(Oryctolagus cuniculus β-globin exon, intron, ファージ P1 (Enterobacteria phage P1) LoxP, マウス(Mus musculus) PGK-1プロモーター, 大腸菌 (Escherichia coli) ネオマイシン耐性遺伝子, SV40(simian virus 40) SV40 polyA, 合成核酸 FLAG, マウス(Mus musculus hnRNPU(heterogeneous nuclear ribonucleoprotein U), ウシ(Bos taurus) bovine growth hormone polyA signal |
Research application | Cre/loxP system |
提供条件 | 条件を付加する。 研究成果の公表にあたって謝辞の表明を必要とする。 |
Depositor | 中川 真一(国立研究開発法人理化学研究所) |
Strain Status | 凍結精子 |
Strain Availability | 受注後、検査を施して提供 |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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