Strain Information | |
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Image | |
BRC No. | RBRC09735 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6N-Tg(CAG-EGFP,-IL6/mCherry)56Kenw |
Former Common name | LGL-IL6 (Li56) |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6NCrSlc |
Appearance | |
Strain development | Developed by Ken Watanabe, National Center for Geriatrics and Gerontology in 2015. C57BL/6N genetic background. |
Strain description | Transgenic mouse line to express Human IL-6 in a Cre-dependent manner. CAG promoter drives loxP-GFP-pA-loxP-IL-6-2A-mCherry-pA. Another transgenic subline of RBRC09735 is available as RBRC09734. |
Colony maintenance | |
References | Generation of a transgenic mouse line for conditional expression of human IL-6. Mori T, Murasawa Y, Ikai R, Hayakawa T, Nakamura H, Ogiso N, Niida S, Watanabe K Exp. Anim., 65(4):455-463 (2016). 27349442 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Mouse Proacrosin signal sequence | UN | ||||||
Thy-1 GPI anchor signal sequence | UN | ||||||
SV40 polyA | UN | ||||||
EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | UN | EGFP | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
GH | Growth hormone polyA (Bovine) | UN | GH | ||||
IL6 | interleukin 6 (human) | UN | IL6 | ||||
T2A | Thosea asigna virus 2A peptide (insect) | UN | T2A | ||||
loxP | phage P1 loxP | UN | loxP | ||||
loxP | phage P1 loxP | UN | loxP | ||||
mCherry | Red Fluorescent Protein (Discosoma sp.) | UN | mCherry |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Bacteriophage loxP sites, Aequorea victoria EGFP cDNA, SV40 PolyA signal, Human IL-6 cDNA, Mouse Proacrosin signal sequence; Thy-1 GPI anchor signal sequence, Thosea asigna virus 2A peptide (T2A), Discosoma sp. mCherry cDNA, Bovine GH polyA signal |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System covid19_onset |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mori et al. Exp. Anim. (2016) |
Depositor | Ken Watanabe (National Center for Geriatrics and Gerontology) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |