Strain Information | |
---|---|
Image | |
BRC No. | RBRC09734 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6N-Tg(CAG-EGFP,-IL6/mCherry)29Kenw |
Former Common name | LGL-IL6 (Li29) |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6NCrSlc |
Appearance | |
Strain development | Developed by Ken Watanabe, National Center for Geriatrics and Gerontology in 2015. C57BL/6N genetic background. |
Strain description | Transgenic mouse line to express Human IL-6 in a Cre-dependent manner. CAG promoter drives loxP-GFP-pA-loxP-IL-6-2A-mCherry-pA. Another transgenic subline of RBRC09734 is available as RBRC09735. |
Colony maintenance | |
References | Generation of a transgenic mouse line for conditional expression of human IL-6. Mori T, Murasawa Y, Ikai R, Hayakawa T, Nakamura H, Ogiso N, Niida S, Watanabe K Exp. Anim., 65(4):455-463 (2016). 27349442 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |||||||
---|---|---|---|---|---|---|---|
Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
SV40 polyA signal | UN | ||||||
mouse Proacrosin signal sequence | UN | ||||||
Thy-1 GPI anchor signal sequence | UN | ||||||
EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | UN | EGFP | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
GH | Growth hormone polyA (Bovine) | UN | GH | ||||
IL6 | interleukin 6 (human) | UN | IL6 | ||||
T2A | Thosea asigna virus 2A peptide (insect) | UN | T2A | ||||
loxP | phage P1 loxP | UN | loxP | ||||
loxP | phage P1 loxP | UN | loxP | ||||
mCherry | Red Fluorescent Protein (Discosoma sp.) | UN | mCherry |
Phenotype | |
---|---|
Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
---|---|
Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Bacteriophage loxP sites, Aequorea victoria EGFP cDNA, SV40 PolyA signal, Human IL6 cDNA, Thosea asigna virus 2A peptide (T2A), Mouse Proacrosin signal sequence, Discosoma sp. mCherry cDNA, Thy-1 GPI anchor signal sequence, Bovine GH polyA signal |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System covid19_onset |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mori et al. Exp. Anim. (2016) |
Depositor | Ken Watanabe (National Center for Geriatrics and Gerontology) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
---|
No Data |