RBRC09716

Information1

Image
BRC No.RBRC09716
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Tmem246<em1(EGFP)Osbir>
Former Common namePgap4+/-
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Taroh Kinoshita and Tetsuya Hirata, Research Institute for Microbial Diseases, Osaka University in 2006. EGR-G101 ES cell line was used. EGFP was inserted to the Tmem246 (Pgap4) gene locus with CRISPR/Cas9 technique. C57BL/6 background.
Strain descriptionEGFP knock-in mice at the Tmem246 (Pgap4) gene locus. EGFP was placed 45 bp downstream of the translation initiation codon.
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6JJcl]
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tmem246transmembrane protein 2464Tmem246<em1(EGFP)Osbir>endonuclease-mediated mutation 1, Taroh Kinoshita

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)4EGFP

Information2

Donor DNAJellyfish EGFP cDNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定。(will be announced).
DepositorTaroh Kinoshita (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Category I MTA: CRISPR/Cas9 genome edited bioresources ( Japanese / English )
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Genotyping protocol -PCR-

BRC mice in Publications

No Data