Strain Data Sheet

RBRC09711

Strain Information

Image
BRC No.RBRC09711
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tg(Arc-tTA/EGFP/Capzb<*>2)100Mkyph
Former Common nameACE-TG, AiCE-Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Maki Yamada, Tokushima Bunri University, Kagawa School of Pharmaceutical Sciences. A DNA construct was injected to fertilized eggs derived from C57BL/6N. Mice were maintained in C57BL/6 background.
Strain descriptionTransgenic mice expressing GFP/Capzb2 fusion protein and tTA under the control of Arc gene promoter (7.0kb). This line has 100 copies of the TG construct.
Colony maintenance
References
Experience-dependent, rapid structural changes in hippocampal pyramidal cell spines.
Kitanishi T, Ikegaya Y, Matsuki N, Yamada M K
Cerebral Cortex,19, 2572-2578 (2009). 19240139

Activity-dependent localization in spines of the F-actin capping protein CapZ screened in a rat model of dementia.
Kitanishi T, Sakai J, Kojima S, Saitoh Y, Inokuchi K, Fukaya M, Watanabe M, Matsuki N, Yamada M K
Genes to Cells, 15, 737-747 (2010). 20545768

Traceable stimulus-dependent rapid molecular changes in dendritic spines in the brain.
Kuboyama K, Inoue T, Hashimotodani Y, Itoh T, Suzuki T, Tetsuzawa A, Ohtsuka Y, Kinoshita R, Takara R, Miyazawa T, Gusain P, Kano M, Yamada M K
Sci. Rep., 10(1):15266 (2020). 32943708

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Capzb capping protein (actin filament) muscle Z-line, betaUNCapzb
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP
P2A porcine teschovirus-1 2A peptideUNP2A
tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTA mouse Arc promoter
SV40 polyA signalUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Arc promoter, tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain; Clontech Tet-Off Advanced Inducible Gene Expression System), P2A (porcine teschovirus-1 2A peptide), jellyfish EGFP cDNA from Clontech EGFP-C1 vector, mouse CapZ cDNA, SV40 polyA signal from Clontech EGFP-C1 vector[update 07/14/2020]
    Research applicationFluorescent Proteins/lacZ System
    Tet system
    Specific Term and Conditions1. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested, referring to the mice as "AiCE-Tg". Sci. Rep., 10(1):15266 (2020).
    2, For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorMaki Yamada (Tokushima Bunri University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Sequence of AiCE-Transgene

    BRC mice in Publications

    No Data