Strain Data Sheet

RBRC09687

Strain Information

Image
BRC No.RBRC09687
TypeChemically-induced Mutation Congenic
SpeciesMus musculus
Strain nameC3.B6-Rnf212<repro57>
Former Common nameRepro57
H-2 Haplotype
ES Cell line
Background strainC3H/HeJJcl
Appearance
Strain developmentDeveloped by the NIH-supported Reproductive Genomics program at JAX. Deposited by Tetsuo Kunieda, Okayama University in 2016. C3H congenic line.
Strain descriptionAn ENU-induced mutant line harboring a nonsense mutation of the 4th exon of the mouse Rnf212 (ring finger protein 212) gene. Homozygous male and female exhibit infertility.
Colony maintenance
References
An ENU-induced mutation in the mouse Rnf212 gene is associated with male meiotic failure and infertility.
Yasuhiro Fujiwara, Hirokazu Matsumoto, Kouyou Akiyama, Anuj Srivastava, Mizuho Chikushi, Mary Ann Handel, Tetsuo Kunieda
Reproduction, 149, 67-74 (2014). 25342176

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rnf212
MGI:3645767		ring finger protein 2125Rnf212<repro57>
MGI:3708256		reproductive mutant 10, JAX Reproductive Mutagenesis Program

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal germ cell morphology (MP:0002208)

  • arrest of male meiosis (MP:0008261)

  • azoospermia (MP:0005159)

  • decreased seminal vesicle weight (MP:0004910)

  • decreased testis weight (MP:0004852)
    • more 1 phenotypes
  • male infertility (MP:0001925)
    • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorTetsuo Kunieda (Okayama University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Ogonuki N, Hino T, Fujiwara Y, Osawa Y, Mizuno S, Sugiyama F, Kunieda T, Otsuki J, Oura S, Furuse T, Okada Y, Tamura M, de la Casa-Esperon E, Ikawa M, Inoue K, Ogura A.
    Spermatocyte injection into meiotic oocytes rescues diplotene, but not pachytene, arrest in azoospermic mutant mice.
    Hum Reprod Open 2025(4) hoaf067(2025) 41268068