Strain Data Sheet

RBRC09685

Strain Information

Image
BRC No.RBRC09685
TypeChemically-induced Mutation Congenic
SpeciesMus musculus
Strain nameC3B6-Mad2l2<repro22>
Former Common namerepro22
H-2 Haplotype
ES Cell line
Background strainC3H/HeJJcl
Appearance
Strain developmentDeveloped by the NIH-supported Reproductive Genomics program at JAX. Deposited by Tetsuo Kunieda, Okayama University in 2016. C57BL/6 and C3H mixed genetic backgrounds.
Strain descriptionAn ENU-induced mutant line harboring a missense mutation in the 3rd exon of the mouse Mad2l2 (MAD2 mitotic arrest deficient-like 2) gene. Amino acid substitution of arginine for cysteine at position 70 (C70R). Both male and female homozygotes exhibit infertility. Female homozygotes develop ovarian tumors at a high frequency.
Colony maintenance
References
Lack of Rev7 function results in development of tubulostromal adenomas in mouse ovary.
Abbasi A, Khalaj M, Akiyama K, Mukai Y, Matsumoto H, Acosta T J, Said N, Yoshida M, Kunieda T
Mol. Cell Endocrinol., 412, 19-25, (2015). 26004212

Leydig cell hyperplasia in an ENU-induced mutant mouse with germ cell depletion.
Khalaj M, Abbasi A R, Nishimura R, Akiyama K, Tsuji T, Noguchi J, Okuda K, Kunieda T
J. Reprod. Dev. 54, 225-228, (2008). 18385543

A missense mutation in Rev7 disrupts formation of Polzeta, impairing mouse development and repair of genotoxic agent-induced DNA lesions.
Khalaj M, Abbasi A, Yamanishi H, Akiyama K, Wakitani S, Kikuchi S, Hirose M, Yuzuriha M, Magari M, Degheidy H A, Abe K, Ogura A, Hashimoto H, Kunieda T
J. Biol. Chem., 289, 3811-3824, (2014). 24356953

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mad2l2MAD2 mitotic arrest deficient-like 24Mad2l2reproductive mutant 22, JAX Reproductive Mutagenesis Program
  • Fanconi anemia complementation group V(MedGEN)
  • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • absent germ cells(MP:0004806)

  • absent ovarian follicles(MP:0002777)

  • arrest of spermatogenesis(MP:0001155)

  • azoospermia(MP:0005159)

  • cellular phenotype(MP:0005384)
  • more 14 phenotypes
  • decreased cell proliferation(MP:0000352)

  • decreased enterocyte cell number(MP:0000339)

  • decreased male germ cell number(MP:0004901)

  • decreased oocyte number(MP:0005431)

  • decreased primordial germ cell number(MP:0008392)

  • decreased testis weight(MP:0004852)

  • embryonic lethality, incomplete penetrance(MP:0011102)

  • female infertility(MP:0001926)

  • increased enterocyte apoptosis(MP:0008885)

  • male infertility(MP:0001925)

  • prenatal growth retardation(MP:0010865)

  • reproductive system phenotype(MP:0005389)

  • small ovary(MP:0001127)

  • small testis(MP:0001147)
  • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorTetsuo Kunieda (Okayama University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data