Strain Information | |
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Image | |
BRC No. | RBRC09666 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Gt(ROSA)26Sor<tm2(CAG-cat,-Fam213b)Watk> |
Former Common name | mPMPGFS KI |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | |
Appearance | |
Strain development | Developed by Kikuko Watanabe, Koshien University in 2016. RENKA ES cell line (C57BL/6N) was used. Mice were maintained in C57BL/6 background. |
Strain description | Knock-in mice for Cre-dependent overexpression of mouse Fam213b. Targeted insertion of CAG-loxP-CAT-FRT-PGK-neo-FRT-loxP-Fam213b-pA cassette into the mouse Rosa26 locus. |
Colony maintenance | |
References | J. Biochem., 150, 593-596 (2011). 21926128 J. Biol. Chem., 283, 792-801 (2008). 18006499 Genes. Dev., 5, 1513-1523 (1991). 1653172 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm2(CAG-cat,-Fam213b)Watk>targeted mutation 2, Kikuko Watanabe Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Fam213bfamily with sequence similarity 213, member B6Fam213b Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter catE. coli chloramphenicol acetyltransferase (CAT)6CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Ordering Information | |
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Donor DNA | マウス(Mus musculus) ROSA26, ウシ(Bos taurus) bovine growth hormone polyA signal, サイトメガロウィルス(cytomegalovirus) CMV promoter, ファージ P1 (Enterobacteria phage P1) Lox-P, 大腸菌(Escherichia coli) CAT((n) chloramphenicol O-acetyltransferase), SV40(simian virus 40) SV40 polyA, 出芽酵母(Saccharomyces cerevisiae) FRT, マウス(Mus musculus)PGK-1プロモーター, 大腸菌 (Escherichia coli)ネオマイシン耐性遺伝子, マウス(Mus musculus) Fam213b |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biochem., 150, 593-596 (2011). J. Biol. Chem., 283, 492-801 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the Dr. Kikuko Watanabe is requested. |
Depositor | Kikuko Watanabe (Kansai Medical University) |
Strain Status | Frozen embryos |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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