Strain Data Sheet

RBRC09666

Strain Information

Image
BRC No.RBRC09666
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm2(CAG-cat,-Fam213b)Watk>
Former Common namemPMPGFS KI
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Kikuko Watanabe, Koshien University in 2016. RENKA ES cell line (C57BL/6N) was used. Mice were maintained in C57BL/6 background.
Strain descriptionKnock-in mice for Cre-dependent overexpression of mouse Fam213b. Targeted insertion of CAG-loxP-CAT-FRT-PGK-neo-FRT-loxP-Fam213b-pA cassette into the mouse Rosa26 locus.
Colony maintenance
References
Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice.
Friedrich G, Soriano P
Genes. Dev., 5, 1513-1523 (1991). 1653172

Molecular characterization of a novel type of prostamide/prostaglandin F synthase, belonging to the thioredoxin-like superfamily.
Moriuchi H, Koda N, Okuda-Ashitaka E, Daiyasu H, Ogasawara K, Toh H, Ito S, Woodward D F, Watanabe K
J. Biol. Chem., 283, 792-801 (2008). 18006499

Recent reports about enzymes related to the synthesis of prostaglandin (PG) F(2) (PGF(2alpha) and 9alpha, 11beta-PGF(2)).
Watanabe K
J. Biochem., 150, 593-596 (2011). 21926128

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fam213b family with sequence similarity 213, member B6Fam213b
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm2(CAG-cat,-Fam213b)Watk> targeted mutation 2, Kikuko Watanabe
cat E. coli chloramphenicol acetyltransferase (CAT)6 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • obsolete no phenotypic analysis (MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAマウス(Mus musculus) ROSA26, ウシ(Bos taurus) bovine growth hormone polyA signal, サイトメガロウィルス(cytomegalovirus) CMV　promoter, ファージ　P1 (Enterobacteria phage P1) Lox-P, 大腸菌(Escherichia coli) CAT((n) chloramphenicol O-acetyltransferase), SV40(simian virus 40) SV40 polyA, 出芽酵母(Saccharomyces cerevisiae) FRT, マウス(Mus musculus)PGK-1プロモーター, 大腸菌 (Escherichia coli)ネオマイシン耐性遺伝子, マウス(Mus musculus) Fam213b
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biochem., 150, 593-596 (2011). J. Biol. Chem., 283, 492-801 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the Dr. Kikuko Watanabe is requested.
    DepositorKikuko Watanabe (Kansai Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data