Strain Data Sheet
RBRC09650
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC09650 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6-Tg(Slc1a2-G-CaMP7)Bsi |
| Former Common name | GLT1-G-CaMP7, G7NG817 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6JJcl |
| Appearance | |
| Strain development | アストロサイトとニューロンの活動をイメージングする目的で開発し、論文発表した(Monai, H. et al. 2016 (in press) Nat Commun.)。C57BL/6 系統の受精卵を用いて作製。 |
| Strain description | GLT-1プロモーターの制御下でカルシウムセンサーのG-CaMP7を発現するトランスジェニックマウス。脳の広域において、大半のアストロサイトと一部のニューロンがG-CaMP7を発現する。 |
| Colony maintenance | |
| References | Proc. Natl. Acad. Sci. USA, 116(22):11010-11019 (2019). 31097598 J. Neurosci., 20;27, 6607-6619 (2007). 17581948 Nat Commun. (2016) 7:11100. DOI: 10.1038/ncomms11100 27000523 PLoS One, 7, e51286, (2012). 23240011 Sci. Rep., 11, 8150 (2021). 33854148 |
Health Report | |
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| Examination Date / Room / Rack | 2024/01/09Room:3-BRack:F 2023/10/10Room:3-BRack:F 2023/07/10Room:3-BRack:F 2023/04/10Room:3-BRack:F |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) siteUNFrt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GCaMP7RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP7mouse Slc1a2 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin polyAUNHBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE |
Ordering Information | |
|---|---|
| 供与核酸 | mouse Slc1a2 genomic DNA, GCaMP7 (RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), woodchuck hepatitis virus WPRE (woodchuck hepatitis virus posttranscriptional regulatory element) sequence, rabbit Beta globin polyA, yeast FRT (flippase recombination target) site, Phage P1 loxP site derived from pBACe3.6 vector, BAC clone RP23-361H22 |
| Research application | Fluorescent Proteins/lacZ System |
| 提供条件 | 条件を付加する。 利用者へは、研究成果の公表にあたって譲渡者の指定する文献を引用することを推奨する。Nat Commun. (2016) 7:11100. DOI: 10.1038/ncomms11100 |
| Depositor | 平瀬 肇（国立研究開発法人理化学研究所） |
| Strain Status |  生体 凍結胚 凍結精子 |
| Strain Availability | 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 生体マウスを3~6ヶ月以内に提供可能 |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Aug 2017 |
BRC mice in Publications |
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Holstein-Rønsbo S, Gan Y, Giannetto MJ, Rasmussen MK, Sigurdsson B, Beinlich FRM, Rose L, Untiet V, Hablitz LM, Kelley DH, Nedergaard M. Glymphatic influx and clearance are accelerated by neurovascular coupling. Nat Neurosci (2023) 37264158 Monai H, Wang X, Yahagi K, Lou N, Mestre H, Xu Q, Abe Y, Yasui M, Iwai Y, Nedergaard M, Hirase H. Adrenergic receptor antagonism induces neuroprotection and facilitates recovery from acute ischemic stroke. Proc Natl Acad Sci U S A 116(22) 11010-11019(2019) 31097598 |