Strain Data Sheet

RBRC09637

Strain Information

Image
BRC No.RBRC09637
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Gt(Rosa)26Sor<tm1.1(CAG-GCaMP6,-mCherry)Shi>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain development
Strain descriptionRBRC09450　B6-Gt(Rosa)26Sor<tm1(CAG-GCaMP6,-mCherry)Shi>とCreマウスとの交配により、loxPで挟まれたneo耐性遺伝子を含む領域を除去し、B6/JJclを交配しCreを除いたマウス。
Colony maintenance
ReferencesPLoS One, 7, e51286, (2012). 23240011

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(CAG-GCaMP6,-mCherry)Shi>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F2Afoot-and-mouth disease virus (FMD) 2A peptide6F2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GCaMP6RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA6GCaMP6CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly Apolyadenylic acid additional signal6poly A

Ordering Information

供与核酸CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Bacteriophage P1 LoxP site, GCaMP6:RSET tag, chicken smooth muscle M13, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, Rat calmodulin (CaM) cDNA(aa.2-148), Foot-and-mouth disease virus (FMDV derived) 2A peptide (F2A), Discosoma sp. mCherry cDNA, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element(WPRE), polyA, mouse Gt(Rosa)26Sor genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。PLoS One, 7(12) e51286 (2012).
本件リソースの提供に先立ち、利用者は事前に寄託者およびRBRC09450の寄託者の提供承諾書による承諾を得る。本件リソースの提供目的は学術機関における学術研究に限る。本件リソースを寄託者の同意なく第三者に提供することはできない。
DepositorRIKEN BRC（国立研究開発法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: FP license of TBUSA
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data