Strain Data Sheet

RBRC09616

Strain Information

Image
BRC No.RBRC09616
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pdgfra<tm1.1(EGFP/cre/ERT2)Hyma>
Former Common namePDGFRα-GFPCreERT2
H-2 Haplotype
ES Cell lineKTPU8 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain development熊本大学CARDの協力の元、発生医学研究所幹細胞誘導分野で作製。ヘテロマウスをC57BL/6と交配
Strain descriptionPDGFRαの遺伝子座にGFPとCreERT2の融合タンパクcDNAをknock-inした。ヘテロでは表現型無し。
Colony maintenance
ReferencesGenesis., 53, 329-336 (2015). 25884589

Health Report

Examination Date / Room / Rack2024/02/19Room:4-ARack:E
2023/11/20Room:4-ARack:E
2023/08/21Room:4-ARack:E
2023/05/22Room:4-ARack:E
2023/02/20Room:4-ARack:E
2023/02/20Room:4-ARack:E
2022/11/21Room:4-ARack:E
2022/08/22Room:4-ARack:E
2022/05/23Room:4-ARack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pdgfraplatelet derived growth factor receptor, alpha polypeptide5Pdgfra<tm1.1GFP/cre/ERT2Hyma>targeted mutation 1.1, Hiroyuki Miwa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)5ERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase5cre

Ordering Information

供与核酸Jellyfish GFP cDNA, Phage P1 Cre recombinase cDNA, human Estrogen receptor binding domain (ERT2)cDNA, yeast FRT (flipase recombination target) site, mouse Pdgfra genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。
Depositor江良　択実（国立大学法人熊本大学）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
生体マウスを1~3ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Sep 2016

BRC mice in Publications

Nawaz A, Bilal M, Fujisaka S, Kado T, Aslam MR, Ahmed S, Okabe K, Igarashi Y, Watanabe Y, Kuwano T, Tsuneyama K, Nishimura A, Nishida Y, Yamamoto S, Sasahara M, Imura J, Mori H, Matzuk MM, Kudo F, Manabe I, Uezumi A, Nakagawa T, Oishi Y, Tobe K.
Depletion of CD206+ M2-like macrophages induces fibro-adipogenic progenitors activation and muscle regeneration.
Nat Commun 13(1) 7058(2022) 36411280

Kimura K, Ramirez K, Nguyen TAV, Yamashiro Y, Sada A, Yanagisawa H.
Contribution of PDGFRα-positive cells in maintenance and injury responses in mouse large vessels.
Sci Rep 11(1) 8683(2021) 33883668