Strain Data Sheet

RBRC09552

Strain Information

Image
BRC No.RBRC09552
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Actb<tm3.1(tetO-GCaMP6)Kftnk>
Former Common nametetO-GCaMP6, B6;129-Actb<tm1(tetO-GCaMP6)Kftnk>/Kftnk(tetO-GCaMP6)
H-2 Haplotype
ES Cell lineG0G1
Background strainC57BL/6JJcl
Appearance
Strain development寄託者が作出。G0G1 (阪大微研の岡部勝先生が樹立, 129とB6のhybrid) で作成。129SvEvTac、C57BL6Jなどとのmixed background。
Strain descriptiontetOプロモーターの制御下にカルシウムインジケーターGCaMP6 (PLoS One. 2012;7(12):e51286)を発現する。tetOカセットがbeta actin遺伝子下流にノックインされている。
Colony maintenance
ReferencesPLoS One, 7, e51286, (2012). 23240011

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Actbactin, beta5Actb<tm1(tetO-GCaMP6)Kftnk>targeted mutation 1, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GCaMP6RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA5GCaMP6E. coli tet operator, cytomegalovirus CMV promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal5

Ordering Information

供与核酸Phage P1 loxP sites, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, Rabbit Beta globin intron, GCaMP6:RSET tag, chicken smooth muscle M13, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, Rat calmodulin (CaM) cDNA(aa.2-148), SV40 polyA signal, yeast FRT (flipase recombination target) site, mouse beta actin genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
提供条件条件を付加しない。
Depositor田中　謙二（慶應義塾大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Yoshida K, Kato D, Sugio S, Takeda I, Wake H.
Activity-dependent oligodendrocyte calcium dynamics and their changes in Alzheimer's disease.
Front Cell Neurosci 17 1154196(2023) 38026691

Moriya S, Yamashita A, Nishi R, Ikoma Y, Yamanaka A, Kuwaki T.
Acute nociceptive stimuli rapidly induce the activity of serotonin and noradrenalin neurons in the brain stem of awake mice.
IBRO Rep 7 1-9(2019) 31194165