Strain Data Sheet

RBRC09525

Strain Information

Image
BRC No.RBRC09525
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Ins1<em1(cre)Utr>
Former Common nameI2AC mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development筑波大学生命科学動物資源センターの杉山文博先生が2014年に開発。C57BL/6J (CRJ)の受精卵においてマイクロインジェクションによるゲノム編集で作製された系統。ファウンダーマウスとC57BL/6Jマウスを交配し、F1を作出。その後、インタークロス(3世代)により維持・繁殖した。
Strain descriptionIns1遺伝子のstopコドン直前にP2A-nls CreをノックインしたバイシストロニックCreドライバーマウス
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/05/20Room:4-ARack:C
2024/04/08Room:3-ARack:D
2024/02/19Room:4-ARack:C
2024/01/09Room:3-ARack:A
2023/11/20Room:4-ARack:C
2023/11/13Room:4-ARack:C
2023/10/10Room:3-ARack:A
2023/08/21Room:4-ARack:C
2023/08/07Room:4-ARack:C
2023/07/10Room:3-ARack:A
2023/05/22Room:4-ARack:C
2023/04/10Room:3-ARack:A
2023/02/20Room:4-ARack:C
2023/01/10Room:3-ARack:A

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HBB2rabbit beta-globin polyA19HBB2
  • diabetes mellitus, permanent neonatal 4(MedGEN)

  • hyperproinsulinemia(MedGEN)

  • maturity-onset diabetes of the young type 10(MedGEN)
    • more 3 Diseases
  • maturity-onset diabetes of the young(DisGeNET)

  • permanent neonatal diabetes mellitus(DisGeNET)

  • type 1 diabetes mellitus 2(MedGEN)
    • Ins1insulin I19Ins1endonuclease-induced mutation 1, University of Tsukuba Laboratory Animal Resource Center
  • diabetes mellitus, permanent neonatal 4(MedGEN)

  • hyperproinsulinemia(MedGEN)

  • maturity-onset diabetes of the young type 10(MedGEN)
    • more 3 Diseases
  • maturity-onset diabetes of the young(DisGeNET)

  • permanent neonatal diabetes mellitus(DisGeNET)

  • type 1 diabetes mellitus 2(MedGEN)
    • P2Aporcine teschovirus-1 2A peptide19P2A
  • diabetes mellitus, permanent neonatal 4(MedGEN)

  • hyperproinsulinemia(MedGEN)

  • maturity-onset diabetes of the young type 10(MedGEN)
    • more 3 Diseases
  • maturity-onset diabetes of the young(DisGeNET)

  • permanent neonatal diabetes mellitus(DisGeNET)

  • type 1 diabetes mellitus 2(MedGEN)
    • crePhage P1 Cre recombinase19cre
  • diabetes mellitus, permanent neonatal 4(MedGEN)

  • hyperproinsulinemia(MedGEN)

  • maturity-onset diabetes of the young type 10(MedGEN)
    • more 3 Diseases
  • maturity-onset diabetes of the young(DisGeNET)

  • permanent neonatal diabetes mellitus(DisGeNET)

  • type 1 diabetes mellitus 2(MedGEN)
    • nlsSV40 large T antigen nuclear localization signal (NLS)19nls
  • diabetes mellitus, permanent neonatal 4(MedGEN)

  • hyperproinsulinemia(MedGEN)

  • maturity-onset diabetes of the young type 10(MedGEN)
    • more 3 Diseases
  • maturity-onset diabetes of the young(DisGeNET)

  • permanent neonatal diabetes mellitus(DisGeNET)

  • type 1 diabetes mellitus 2(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    供与核酸P2A (porcine teschovirus-1 2A peptide), Simian virus 40 nuclear localization signal (NLS), bacteriophage P1 Cre recombinase, rabbit beta globin polyA
    Research applicationCre/loxP system
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。(will be announced.)
    Depositor杉山　文博（国立大学法人筑波大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Dec 2018

    BRC mice in Publications

    Mostafa D, Yanagiya A, Georgiadou E, Wu Y, Stylianides T, Rutter GA, Suzuki T, Yamamoto T.
    Loss of β-cell identity and diabetic phenotype in mice caused by disruption of CNOT3-dependent mRNA deadenylation.
    Commun Biol 3(1) 476(2020) 32859966