Strain Data Sheet

RBRC09485

Strain Information

Image
BRC No.RBRC09485
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Xcr1<tm2(HBEGF/Venus)Ksho>
Former Common nameXCR1-DTRvenus
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain development改正恒康先生（理化学研究所　統合生命医科学研究センター　炎症制御研究チーム　客員主管研究員）が理化学研究所　免疫・アレルギー科学総合研究センターにて2008年頃作製した。C57BL/6 コンジェニック系統。C57BL/6JJclに12世代戻し交配を行った。Y染色体は原系統。Developed by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2008.
Strain descriptionケモカイン受容体XCR1は、ケモカインXCL1の受容体であり、主として樹状細胞の一つのサブセットに高い特異性をもって発現している。本マウスは、XCR1遺伝子座にヒト由来ジフテリア毒素受容体 (DTR (HBEGF) ) と蛍光タンパク質venus (理研BSI宮脇敦史先生由来) との融合タンパク質 (DTRvenus) をコードする遺伝子をノックインしたマウスである。Venusの発現にて、XCR1陽性細胞を同定できると同時に、ジフテリア毒素の投与により一時的に生体においてXCR1陽性細胞を除去できる。A chemokine receptor, XCR1, is a receptor for XCL1 and expressed dominantly in one dendritic cell subset. XCR1-DTRvenus mice were generated by knocking the gene encoding a fusion protein consisting of human diphteria toxin receptor (DTR or HBEGF) and a fluorescence protein, venus (derived from Dr. Atsushi Miyawaki at BRI) into the murine XCR1 gene locus. In XCR1-DTRvenus mice, XCR1-expressing cells can be detected by venus expression and ablated transiently by the administration of diphteria toxin.
Colony maintenance
References
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.
Takeharu Nagai, Keiji Ibata, Eun Sun Park, Mie Kubota, Katsuhiko Mikoshiba, Atsushi Miyawaki
Nat. Biotechnol., 20, 87-90 (2002). 11753368

Critical roles of a dendritic cell subset expressing a chemokine receptor, XCR1.
Chihiro Yamazaki, Masanaka Sugiyama, Tomokazu Ohta, Hiroaki Hemmi, Eri Hamada, Izumi Sasaki, Yuri Fukuda, Takahiro Yano, Mikako Nobuoka, Takeshi Hirashima, Akihiko Iizuka, Katsuaki Sato, Takashi Tanaka, Katsuaki Hoshino, Tsuneyasu Kaisho
J. Immunol., 190, 6071-6082 (2013). 23670193

Health Report

Examination Date / Room / Rack2026-07-27Room:4-ARack:G囮検査
2026-05-11Room:4-ARack:G囮検査
2026-03-02Room:4-ARack:G囮検査
2025-12-22Room:4-ARack:G囮検査
2025-10-14Room:4-ARack:G囮検査
2025-07-28Room:4-ARack:G囮検査
2025-05-19Room:4-ARack:G囮検査
2025-02-17Room:4-ARack:G囮検査
2024-11-18Room:4-ARack:G囮検査
2024-11-06Room:4-ARack:G追加検査

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site9Frt
Frt yeast FRT (flippase recombination target) site9Frt
GH Growth hormone polyA (Bovine)9GH
HBEGF heparin-binding EGF-like growth factor (human)9HBEGF
Venus Yellow Fluorescent Protein (Aequorea victoria)9Venus
Xcr1
MGI:1346338		chemokine (C motif) receptor 19Xcr1<tm2(HBEGF/Venus)Ksho>
MGI:5544058		targeted mutation 2, Tsuneyasu Kaisho
neo neomycin resistance gene (E. coli)9 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal CD8-positive, alpha-beta T cell physiology (MP:0004392)

  • abnormal antigen presentation (MP:0001835)

  • decreased dendritic cell number (MP:0008127)

  • decreased interferon-gamma secretion (MP:0008567)

  • decreased susceptibility to bacterial infection (MP:0002411)
    • Detailed phenotype data

    Ordering Information

    供与核酸human HB-EGF (DTR) cDNA, Aequorea coerulescens mutant GFP (venus) cDNA, Bos taurus bGH polyA, Saccharomyces cerevisiae FRT sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), Escherichia coli Neomycin resistance gene, HSV TK polyA signal, mouse Xcr1 genomic DNA
    Research applicationFLP/frt system
    Fluorescent Proteins/lacZ System
    Immunology and Inflammation Research
    提供条件利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。J. Immunol., 190, 6071-6082 (2013). 学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。研究成果の公表にあたってVenusの開発者である宮脇敦史先生への謝辞の表明並びに指定する文献を引用する。Nat. Biotechnol., 20, 87-90 (2002).
    Depositor改正　恒康（国立研究開発法人理化学研究所）
    Strain Status生体のアイコン生体
    凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    生体マウスを1~3ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jan 2016

    BRC mice in Publications

    Takahashi S, Inoue T, Ensbey KS, Legg SRW, Sekiguchi T, Nelson E, Nemychenkov NS, Joshi T, Minnie SA, Yeh AC, Zhang P, Headley M, Paik J, Amory JK, Hill GR, Koyama M.
    Targeting donor XCR1+ and CD11b+ dendritic cells prevents Th1- and Th17-dependent GVHD within the gastrointestinal tract.
    Blood 147(18) 2143-2155(2026) 41662631
    Shamskhou EA, Duffy FJ, Cross LM, Plumlee CR, Gern BH, Barrett HW, Diercks AH, Cohen SB, Sivakumar R, Aitchison JD, Ganusov VV, Urdahl KB, Gerner MY.
    Monocytic niches escape T cell surveillance and promote Mycobacterium tuberculosis persistence in lymph nodes.
    Immunity (2026) 42335893
    Hogg GD, Weinstein AG, Kingston NL, Liu X, Dres OM, Kang LI, Lander VE, Kao YL, Ahmad F, Knolhoff BL, Shenoy VV, Sells B, Jayasinghe RG, Houston A, Liu T, Herndon JM, Murphy KM, Ding L, Fields RC, Panni RZ, Hawkins WG, DeNardo DG.
    Combined Flt3L and CD40 agonism restores dendritic cell-driven T cell immunity in pancreatic cancer.
    Sci Immunol 10(110) eadp3978(2025) 40815670
    Meiser P, Knolle MA, Hirschberger A, de Almeida GP, Bayerl F, Lacher S, Pedde AM, Flommersfeld S, Hönninger J, Stark L, Stögbauer F, Anton M, Wirth M, Wohlleber D, Steiger K, Buchholz VR, Wollenberg B, Zielinski CE, Braren R, Rueckert D, Knolle PA, Kaissis G, Böttcher JP.
    A distinct stimulatory cDC1 subpopulation amplifies CD8+ T cell responses in tumors for protective anti-cancer immunity.
    Cancer Cell 41(8) 1498-1515.e10(2023) 37451271
    Zagorulya M, Yim L, Morgan DM, Edwards A, Torres-Mejia E, Momin N, McCreery CV, Zamora IL, Horton BL, Fox JG, Wittrup KD, Love JC, Spranger S.
    Tissue-specific abundance of interferon-gamma drives regulatory T cells to restrain DC1-mediated priming of cytotoxic T cells against lung cancer.
    Immunity 56(2) 386-405.e10(2023) 36736322
    Inui H, Nishida M, Ichii M, Nakaoka H, Asaji M, Ide S, Saito S, Saga A, Omatsu T, Tanaka K, Kanno K, Chang J, Zhu Y, Okada T, Okuzaki D, Matsui T, Ohama T, Koseki M, Morii E, Hosen N, Yamashita S, Sakata Y.
    XCR1+ conventional dendritic cell-induced CD4+ T helper 1 cell activation exacerbates cardiac remodeling after ischemic myocardial injury.
    J Mol Cell Cardiol 176 68-83(2023) 36739942
    de Mingo Pulido Á, Hänggi K, Celias DP, Gardner A, Li J, Batista-Bittencourt B, Mohamed E, Trillo-Tinoco J, Osunmakinde O, Peña R, Onimus A, Kaisho T, Kaufmann J, McEachern K, Soliman H, Luca VC, Rodriguez PC, Yu X, Ruffell B.
    The inhibitory receptor TIM-3 limits activation of the cGAS-STING pathway in intra-tumoral dendritic cells by suppressing extracellular DNA uptake.
    Immunity 54(6) 1154-1167.e7(2021) 33979578