Strain Data Sheet

RBRC09485

Strain Information

Image
BRC No.RBRC09485
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Xcr1<tm2(HBEGF/Venus)Ksho>
Former Common nameXCR1-DTRvenus
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2008.
Strain descriptionA chemokine receptor, XCR1, is a receptor for XCL1 and expressed dominantly in one dendritic cell subset. XCR1-DTRvenus mice were generated by knocking the gene encoding a fusion protein consisting of human diphteria toxin receptor (DTR or HBEGF) and a fluorescence protein, venus (derived from Dr. Atsushi Miyawaki at BRI) into the murine XCR1 gene locus. In XCR1-DTRvenus mice, XCR1-expressing cells can be detected by venus expression and ablated transiently by the administration of diphteria toxin.
Colony maintenance
References
Critical roles of a dendritic cell subset expressing a chemokine receptor, XCR1.
Chihiro Yamazaki, Masanaka Sugiyama, Tomokazu Ohta, Hiroaki Hemmi, Eri Hamada, Izumi Sasaki, Yuri Fukuda, Takahiro Yano, Mikako Nobuoka, Takeshi Hirashima, Akihiko Iizuka, Katsuaki Sato, Takashi Tanaka, Katsuaki Hoshino, Tsuneyasu Kaisho
J. Immunol., 190, 6071-6082 (2013). 23670193

Health Report

Examination Date / Room / Rack2025/12/22Room:4-ARack:GSentinel mouse program
2025/10/14Room:4-ARack:GSentinel mouse program
2025/07/28Room:4-ARack:GSentinel mouse program
2025/05/19Room:4-ARack:GSentinel mouse program
2025/02/17Room:4-ARack:GSentinel mouse program
2024/11/18Room:4-ARack:GSentinel mouse program
2024/11/06Room:4-ARack:GAdditional tests
2024/08/19Room:4-ARack:GSentinel mouse program
2024/05/20Room:4-ARack:GSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site9Frt
Frt yeast FRT (flippase recombination target) site9Frt
GH Growth hormone polyA (Bovine)9GH
HBEGF heparin-binding EGF-like growth factor (human)9HBEGF
Venus Yellow Fluorescent Protein (Aequorea victoria)9Venus
Xcr1
MGI:1346338		chemokine (C motif) receptor 19Xcr1<tm2(HBEGF/Venus)Ksho>
MGI:5544058		targeted mutation 2, Tsuneyasu Kaisho
neo neomycin resistance gene (E. coli)9 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal CD8-positive, alpha-beta T cell physiology (MP:0004392)

  • abnormal antigen presentation (MP:0001835)

  • decreased dendritic cell number (MP:0008127)

  • decreased interferon-gamma secretion (MP:0008567)

  • decreased susceptibility to bacterial infection (MP:0002411)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman HB-EGF (DTR) cDNA, Aequorea coerulescens mutant GFP (venus) cDNA, Bos taurus bGH polyA, Saccharomyces cerevisiae FRT sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), Escherichia coli Neomycin resistance gene, HSV TK polyA signal, mouse Xcr1 genomic DNA
    Research applicationFLP/frt system
    Fluorescent Proteins/lacZ System
    Immunology and Inflammation Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 190, 6071-6082 (2013). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorTsuneyasu Kaisho (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jan 2016

    BRC mice in Publications

    Hogg GD, Weinstein AG, Kingston NL, Liu X, Dres OM, Kang LI, Lander VE, Kao YL, Ahmad F, Knolhoff BL, Shenoy VV, Sells B, Jayasinghe RG, Houston A, Liu T, Herndon JM, Murphy KM, Ding L, Fields RC, Panni RZ, Hawkins WG, DeNardo DG.
    Combined Flt3L and CD40 agonism restores dendritic cell-driven T cell immunity in pancreatic cancer.
    Sci Immunol 10(110) eadp3978(2025) 40815670
    Meiser P, Knolle MA, Hirschberger A, de Almeida GP, Bayerl F, Lacher S, Pedde AM, Flommersfeld S, Hönninger J, Stark L, Stögbauer F, Anton M, Wirth M, Wohlleber D, Steiger K, Buchholz VR, Wollenberg B, Zielinski CE, Braren R, Rueckert D, Knolle PA, Kaissis G, Böttcher JP.
    A distinct stimulatory cDC1 subpopulation amplifies CD8+ T cell responses in tumors for protective anti-cancer immunity.
    Cancer Cell 41(8) 1498-1515.e10(2023) 37451271
    Zagorulya M, Yim L, Morgan DM, Edwards A, Torres-Mejia E, Momin N, McCreery CV, Zamora IL, Horton BL, Fox JG, Wittrup KD, Love JC, Spranger S.
    Tissue-specific abundance of interferon-gamma drives regulatory T cells to restrain DC1-mediated priming of cytotoxic T cells against lung cancer.
    Immunity 56(2) 386-405.e10(2023) 36736322
    Inui H, Nishida M, Ichii M, Nakaoka H, Asaji M, Ide S, Saito S, Saga A, Omatsu T, Tanaka K, Kanno K, Chang J, Zhu Y, Okada T, Okuzaki D, Matsui T, Ohama T, Koseki M, Morii E, Hosen N, Yamashita S, Sakata Y.
    XCR1+ conventional dendritic cell-induced CD4+ T helper 1 cell activation exacerbates cardiac remodeling after ischemic myocardial injury.
    J Mol Cell Cardiol 176 68-83(2023) 36739942
    de Mingo Pulido Á, Hänggi K, Celias DP, Gardner A, Li J, Batista-Bittencourt B, Mohamed E, Trillo-Tinoco J, Osunmakinde O, Peña R, Onimus A, Kaisho T, Kaufmann J, McEachern K, Soliman H, Luca VC, Rodriguez PC, Yu X, Ruffell B.
    The inhibitory receptor TIM-3 limits activation of the cGAS-STING pathway in intra-tumoral dendritic cells by suppressing extracellular DNA uptake.
    Immunity 54(6) 1154-1167.e7(2021) 33979578