Strain Data Sheet

RBRC09479

Strain Information

Image
BRC No.RBRC09479
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameA.129X-Ryr2<tm1(CAG-KRAS<*>G12V,-lacZ)Nobs>
Former Common nameA/J-Ryr2<tm1Nobs>
H-2 Haplotype
ES Cell lineRW4 [129X1/SvJ]
Background strain
Appearance
Strain development開発者；鈴木昇先生、2001年
Strain descriptionRyr2遺伝子の第一イントロンに,CAG‐Lox‐stop‐lox‐ human KrasG12V‐IRES‐LacZ‐polyAをノックイン
Colony maintenance
ReferencesBiochem. Biophys. Res. Commun., 452(4):1067-70 (2014). 25245290
Oncogene., 25, 7673-7679 (2006). 16785989

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ryr2ryanodine receptor 2, cardiac13Ryr2<tm1(CAG-KRAS*G12V,-lacZ)Nobs>targeted mutation 1, Noboru Suzuki9330127I20Rik

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)13GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
KRASKirsten rat sarcoma viral oncogene homolog (KRAS)13KRAS

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)13

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)13nls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)13puroCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA13

Ordering Information

供与核酸CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, yeast HIS3 DNA, Streptomyces alboniger puromycin resistant gene, human K-rasG12V cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), SV40 nuclear localizing signal, E. coli lacZ, bovine growth hormone polyadenylation signal, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 poly A additional signal, mouse Ryr2 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。
Depositor鈴木　昇（国立大学法人三重大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data