Strain Data Sheet

RBRC09435

Strain Information

Image
BRC No.RBRC09435
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Gt(ROSA)26Sor<tm1(Stop-3xFlag ESET,EGFP)>
Former Common nameRosa26-3xFlag-ESET-IRES-GFP- KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development129/B6 F1 ES細胞から作製され以後C57BL/6にbackcrossを6回行った。造血系に発現させた場合には明らかな表現系は認められないが、他に関しては調べていない。
Strain descriptionEset/Setdb1を組織・細胞特異的に発現させる。
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(Stop-3xFlag ESET,EGFP)>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ESET6ESET

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)6FLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

供与核酸Bacteriophage loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, Stop (synthetic), FLAG tag (synthetic), mouse ESET cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Aequorea victoria EGFP cDNA, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって謝辞の表明を必要とする。
Depositor岩間　厚志（国立大学法人千葉大学）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data