Strain Data Sheet

RBRC09393

Strain Information

Image
BRC No.RBRC09393
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Kcnj11<tm2Sse>
Former Common nameKir6.2-flox
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain development神戸大学・清野進先生C57Bl/6由来ES細胞を用いて作出。
Strain descriptionKCNJ11 (Kir6.2)はATP感受性カリウムチャネルを構成するサブユニットのひとつであり、この遺伝子の欠損は膵β細胞におけるインスリン分泌や神経細胞におけるグルコース感受性に異常を来たす。適切なプロモーターによるCreリコンビナーゼの発現によって、組織特異的遺伝子欠損が期待される。
Colony maintenance
ReferencesJ. Clin. Invest., 130(12):6639-6655 (2020). 33196462

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kcnj11potassium inwardly rectifying channel, subfamily J, member 117Kcnj11<tm2Sse>targeted mutation 2, Susumu SeinoKir6.2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)7GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

供与核酸Phage P1 loxP sites, Yeast FRT (flippase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, Bovine Growth hormone poly A signal, mouse Kcnj11 genomic DNA
Research applicationCre/loxP system
Endocrine Deficiency Research
FLP/frt system
Neurobiology Research
提供条件条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。
Depositor清野　進（国立大学法人神戸大学）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data