Strain Data Sheet

RBRC09391

Strain Information

Image
BRC No.RBRC09391
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Rapgef4<tm1Sse>
Former Common nameEpac2-flox
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
References
Essential role of Epac2/Rap1 signaling in regulation of insulin granule dynamics by cAMP.
Tadao Shibasaki, Harumi Takahashi, Takashi Miki, Yasuhiro Sunaga, Kimio Matsumura, Mami Yamanaka, Changliang Zhang, Atsuko Tamamoto, Takaya Satoh, Jun-Ichi Miyazaki, Susumu Seino
Proc. Natl. Acad. Sci. USA, 104:19333, (2007). 18040047

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rapgef4
MGI:1917723		Rap guanine nucleotide exchange factor (GEF) 42Rapgef4<tm1Sse> targeted mutation 1, Susumu Seino
loxP phage P1 loxP2loxP
loxP phage P1 loxP2loxP
loxP phage P1 loxP2loxP
neo neomycin resistance gene (E. coli)2 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal insulin secretion (MP:0003564)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Rapgef4 genomic DNA
    Research applicationCre/loxP system
    Endocrine Deficiency Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorSusumu Seino (Kobe University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data