Strain Data Sheet

RBRC09365

Strain Information

Image
BRC No.RBRC09365
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Gt(ROSA)26Sor<tm5(ACTB-tTA)Luo>Igs7<tm92.1(tetO-YCX2.60)Hze>/J
Former Common nameROSA26-ZtTA;Ai92(TITL-YCX2.60)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development2013年Allen Institute for Brain Scienceにて作製。Ai92(TITL-YCX2.60)(B6;129S-Igs7tm92.1(tetO-YCX2.60)Hze/J)マウスとROSA26-ZtTAマウス(Jackson Lab #012266)を交配。
Strain descriptionCreマウスとの交配によりYCX2.60発現マウスが得られる。
Colony maintenance
ReferencesPLoS One., 5(7):e11503 (2010). 20634890
Neuron., 85(5): 942-958 (2015). 25741722

Health Report

Examination Date / Room / Rack2022/11/07Room:2-検Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm5(ACTB-tTA)Luo>targeted mutation 5, Liqun Luo

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
b-geolacZ reporter gene and neomycin resistance gene6b-geo

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)6tTA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Igs7intergenic site 79Igs7<tm92.1(tetO-YCX2.60)Hze>targeted mutation 92.1, Hongkui Zeng

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F3yeast FRT (flippase recombination target) site (synthetic) variant9F3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F5yeast FRT (flippase recombination target) site (synthetic) variant9F5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)9GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)9GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hyghygromycin phasphotransferase (E. coli)9Hygmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)9WPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
YCX2.60chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated ECFP (cpECFP) cDNA, rat calmodulin (CaM) cDNA, circularly permutated Venus enhanced yellow fluorescent protein cDNA9YCX2.60

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal9

Ordering Information

供与核酸Cytomegalovirus CMV enhancer, chicken β-actin promoter, P1 phage loxP sites, E. coli β-galactosidase, E. coli neomycin-resistance gene, Tet transactivator (E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain), SV40 polyA,/ yeast FRT (flipase recombination target) sites (FRT3 and FRT5), chicken β-globin HS4 insulater, E. coli tetO promoter, synthetic polyA, human hGH polyA, mouse PGK polyA, jellyfish CFP/YFP cDNA, Xenopus CaM, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), bovine bGH polyA, E. coli AttB, λphage AttP, mouse PGK promoter, E. coli hygromycin resistance gene
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
提供条件条件を付加する。利用者は事前に寄託者(開発者)の提供承諾書を得る。
研究成果の公表にあたって寄託者の指定する文献を引用する。Neuron., 85(5): 942-958 (2015).
学術研究に限る。研究成果の公表にあたって謝辞を表明し、発表内容のコピーを寄託者に送る。
Depositor宮脇　敦史（国立研究開発法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-
Sequence of YCX2.60

BRC mice in Publications

No Data