Strain Data Sheet

RBRC09260

Strain Information

Image
BRC No.RBRC09260
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Cnga2<tm1Kob>
Former Common nameCNGA2flox
H-2 Haplotype
ES Cell lineEGR-05 [129S2]
Background strain
Appearance
Strain development大阪バイオサイエンス研究所神経機能学部門・小早川令子先生らにより開発
Strain descriptionCyclic nucleotide-gated channel alpha 2遺伝子のfloxedマウス。エクソン6がloxPで挟まれている。creマウスとの交配により組織特異的なノックアウトマウスを作成可能。
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, (2015). 25564662

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cnga2cyclic nucleotide gated channel alpha 2XCnga2<tm1Kob>targeted mutation 1, Reiko KobayakawaCnca, Cncg4, OCNC1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)Xmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
polyAX

Ordering Information

供与核酸Phaga P1 loxP sites, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene, polyA, mouse Cyclic nucleotide-gated channel alpha 2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Proc. Natl. Acad. Sci. USA, 2015
利用者は、事前に開発者(小早川 令子博士)に連絡して利用の許可を得る。
Depositor小早川 令子(大阪バイオサイエンス研究所)
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Mar 2017

BRC mice in Publications

Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, Kobayakawa K, Saito T, Imai T.
Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice.
Dev Cell (2023) 37290446