Strain Data Sheet

RBRC09256

Strain Information

Image
BRC No.RBRC09256
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm1.1(CAG-kikGR)Kgwa>
Former Common nameB6-ROSA/kikGR KI, B6-ROSA/kikGR KI
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain development京都大学大学院医学研究科・戸村道夫。M1 ES細胞を用いて作出。CAG-Creマウスとの交配により、neoカセット除去済み。C57BL/6コンジェニック。
Strain descriptiongreen-to-red photoconvertible fluorescence protein, KikGRを発現するROSA26ノックインしたマウス。
Colony maintenanceHomozygote x Homozygote [or crossing to C57BL/6JJcl]
References
Efficient selection for high-expression transfectants with a novel eukaryotic vector.
H Niwa, K Yamamura, J Miyazaki
Gene, 108(2):193-9 (1991). 1660837

Tracking and quantification of dendritic cell migration and antigen trafficking between the skin and lymph nodes.
Michio Tomura, Akihiro Hata, Satoshi Matsuoka, Francis H W Shand, Yasutaka Nakanishi, Ryoyo Ikebuchi, Satoshi Ueha, Hidekazu Tsutsui, Kayo Inaba, Kouji Matsushima, Atsushi Miyawaki, Kenji Kabashima, Takeshi Watanabe, Osami Kanagawa
Sci. Rep., e6030 (2014). 25112380

Health Report

Examination Date / Room / Rack2026-08-31Room:3-BRack:J囮検査
2026-06-22Room:3-BRack:J囮検査
2026-04-06Room:3-BRack:J囮検査
2026-01-19Room:3-BRack:C囮検査
2025-11-10Room:3-BRack:C囮検査
2025-09-01Room:3-BRack:C囮検査
2025-06-16Room:3-BRack:C囮検査
2025-04-07Room:3-BRack:C囮検査
2025-01-07Room:3-BRack:C囮検査

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(CAG-kikGR)Kgwa> targeted mutation 1.1, Osami Kanagawa
kikGR Green-Red Fluorescent Protein (Favia favus)6kikGR CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
loxP phage P1 loxP6loxP

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • normal phenotype (MP:0002873)
    • Detailed phenotype data

    Ordering Information

    供与核酸CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP site, Favia favus Kikume-Green Red cDNA, mouse Gt(ROSA)26Sor genomic region
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。Sci. Rep., e6030 (2014). 研究成果の公表にあたって謝辞の表明を必要とする。学術機関の学術研究に限る。営利機関の利用希望者は事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者は当該発表物1部を寄託者に送付する。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
    研究成果の公表にあたってKikGRの開発者である宮脇敦史先生への謝辞の表明並びに指定する文献を引用する。PLoS One 3 (12): e3944 (2008).
    Depositor古関　明彦（独立行政法人理化学研究所統合生命医科学研究センター）
    Strain Status生体のアイコン生体
    凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    生体マウスを3~6ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Ji Z, Gao J, Zhang S, Li J, Wu H, Yao J, Ma X, Xin Y, Zhu Y, Zhao M, Zhao Z, Shen K, Wu T, Qian X, Wang J, An H, Li Y, Sun W, Zhao Q, Zhou X, Gao R, Duan Q, Li C, Geng X, Yang M, Xiao R, Liu J, Wang W, Wang J, Fu Y, Zhang JR, Chen X, Tong P, Cheng G, Qi H, Wu L, Zeng W, Xi Q, Zhang L, Lai Y, Yang W, Zhang Y, Lu Q, Liu W.
    A metabolic alarmin from keratinocytes potentiates systemic humoral immunity.
    Nature (2026) 41781621
    Zhang X, Huang N, Mu Y, Chen H, Zhu M, Zhang S, Liu P, Zhang H, Deng H, Feng K, Shang Q, Liu X, Zhang C, Shi M, Yang L, Sun J, Kong G, Geng J, Lu S, Li Z.
    Mechanical Force-Induced cGAS Activation in Carcinoma Cells Facilitates Splenocytes into Liver to Drive Metastasis.
    Adv Sci (Weinh) 12(8) e2401127(2025) 39737867
    Yu D, Gao X, Shao F, Liu Z, Liu A, Zhao M, Tang Z, Guan Y, Wang S.
    Antigen-presenting innate lymphoid cells induced by BCG vaccination promote a respiratory antiviral immune response through the skin‒lung axis.
    Cell Mol Immunol 22(4) 390-402(2025) 39962263
    Nina Yi-Tzu Lin, Shota Fukuoka, Shohei Koyama, Daisuke Motooka, Dieter M. Tourlousse, Yuko Shigeno, Yuki Matsumoto, Hiroyuki Yamano, Kazutoshi Murotomi, Hideyuki Tamaki, Takuma Irie, Eri Sugiyama, Shogo Kumagai, Kota Itahashi, Tokiyoshi Tanegashima, Kaori Fujimaki, Sachiko Ito, Mariko Shindo, Takahiro Tsuji, Hiroaki Wake, Keisuke Watanabe, Yuka Maeda, Tomohiro Enokida, Makoto Tahara, Riu Yamashita, Takao Fujisawa, Motoo Nomura, Akihito Kawazoe, Koichi Goto, Toshihiko Doi, Kohei Shitara, Hiroyuki Mano, Yuji Sekiguchi, Shota Nakamura, Yoshimi Benno, Hiroyoshi Nishikawa
    Microbiota-driven antitumour immunity mediated by dendritic cell migration
    Nature (2025) 40659786
    De Ponte Conti B, Marino R, Rezzonico-Jost T, Forcato M, Mangani D, Notario E, Gargari G, Carelli E, Rinaldi A, Raimondi A, Moro S, Marzano M, Visci G, Perruzza L, Raneri M, Dallavalle D, Mantegazza G, Montani L, Prisco F, Takur R, Geginat J, Seehusen F, Notarbartolo S, Pesole G, Bicciato S, Guglielmetti S, Grassi F.
    Secretory IgA amplification during immune checkpoint blockade enhances the control of tumor growth by enterotropic T cells.
    Sci Adv 11(40) eaeb5308(2025) 41042869
    Zhang S, Wan D, Zhu M, Wang G, Zhang X, Huang N, Zhang J, Zhang C, Shang Q, Zhang C, Liu X, Liang F, Zhang C, Kong G, Geng J, Yao L, Lu S, Chen Y, Li Z.
    CD11b + CD43 hi Ly6C lo splenocyte-derived macrophages exacerbate liver fibrosis via spleen-liver axis.
    Hepatology 77(5) 1612-1629(2023) 36098707
    Li J, Xia N, Li D, Wen S, Qian S, Lu Y, Gu M, Tang T, Jiao J, Lv B, Nie S, Hu D, Liao Y, Yang X, Shi G, Cheng X.
    Aorta Regulatory T Cells with a Tissue-Specific Phenotype and Function Promote Tissue Repair through Tff1 in Abdominal Aortic Aneurysms.
    Adv Sci (Weinh) 9(9) e2104338(2022) 35332699