Strain Information | |
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Image | |
BRC No. | RBRC06549 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Nodal<tm3.1Hmd> |
Former Common name | Nodal RAREn-flox, B6-Nodal/RAREn floxed |
H-2 Haplotype | |
ES Cell line | G4 [(129S6/SvEvTac x C57BL/6NCr)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Masayuki Uehara and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2009. G4 ES cells derived from (129 x C57BL/6)F1 were used to generate the mutant mice. A frt flanked PGK-neo cassette was removed by crossing with CAG-FLPe mice (B6/129 background). C57BL/6 and 129 mixed background. |
Strain description | Nodal gene REREn floxed mice. REREn in intron 1 of the Nodal gene was flanked by loxP sites. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Genes Dev., 23, 1689-1698 (2009). 19605690 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site10Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP10loxP |
Ordering Information | |
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Donor DNA | phage P1 loxP site, yeast FRT (flipase recombination target) site, mouse Nodal genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev., 23, 1689-1698 (2009). |
Depositor | Hiroshi Hamada (RIKEN CDB) |
Strain Status | Frozen embryos |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |