Strain Data Sheet

RBRC06539

Strain Information

Image
BRC No.RBRC06539
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Dchs2<tm1.1Hmd>
Former Common nameDachsous2 flox, B6-Dchs2 floxed
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Katsura Minegishi and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2012. G4 ES cells derived from (129 x C57BL/6)F1 were used to generate the mutant mice. A frt flanked PGK-neo cassette was removed by crossing with CAG-FLPe mice (B6/129 background). C57BL/6 and 129 mixed background.
Strain descriptionDchs2 (dachsous 2) gene floxed mice. Exon 29 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesDevelopment, 149(9):dev200315 (2022). doi: 10.1242/dev.200315 35420656

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dchs2dachsous 2 (Drosophila)3Dchs2<tm1.1Hmd>targeted mutation 1.1, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Dchs2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 149(9):dev200315 (2022).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Live miceLive mice
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data