Strain Data Sheet

RBRC06511

Strain Information

Image
BRC No.RBRC06511
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Gt(ROSA)26Sor<tm1(Fucci2aR)Jkn>
Former Common nameR26Fucci2aR(RM185R), B6-ROSA26/Fucci2aR KI
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Richard Mort, MRC IGMM, University of Edinburgh in 2014. The mutant mice were corssed to C57BL/6. C57BL/6 mixed background.
Strain descriptionFucci2aR ROSA26 knock-in mice. Targeted insertion of CAG-loxP-Neo-SV40 pA-loxP-Fucci2aR-bovine GH pA cassette into the mouse Rosa26 locus.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J]
References
Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice.
Mort R L, Ford M J, Sakaue-Sawano A, Lindstrom N O, Casadio A, Douglas A T, Keighren M A, Hohenstein P, Miyawaki A, Jackson I J
Cell Cycle, 13, 2681-2696 (2014). 25486356

Health Report

Examination Date / Room / Rack2024/04/22Room:3-CRack:K
2024/01/22Room:3-CRack:K
2023/10/23Room:3-CRack:K
2023/07/24Room:3-CRack:K
2023/04/24Room:3-CRack:K
2023/02/13Room:3-CRack:K
2023/01/23Room:3-CRack:K
2022/10/25Room:3-CRack:K

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA6
CDT1chromatin licensing and DNA replication factor (human)6CDT1
GHGrowth hormone polyA (Bovine)6GH
GMNNgeminin (human)6GMNN
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sortargeted mutation 1, Ian Jackson
T2AThosea asigna virus 2A peptide (insect)6T2A
loxPphage P1 loxP6loxP
loxPphage P1 loxP6loxP
mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry
mVenusYellow Fluorescent Protein (Aequorea victoria)6mVenus
neoneomycin resistance gene (E. coli)6CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • neonatal lethality, complete penetrance(MP:0011087)
  • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), P1 phage loxP sites, E. coli neo, SV40 polyA, human CDT1 cDNA, Discosoma sp. mCherry cDNA, Thosea asigna virus 2A peptide (T2A), Jellyfish variant of enhanced yellow fluorescent protein (mVenus) cDNA, human GMNN cDNA, bovine GH polyA, mouse Rosa26 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
    2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature(s) designated by the DEPOSITOR is requested. Cell Cycle 13:17, 2681-2696, 2014.
    3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    5) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorRichard Mort (The University of Edinburgh)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. UCSD MTA
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data