Strain Data Sheet
RBRC06511
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC06511 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129-Gt(ROSA)26Sor<tm1(Fucci2aR)Jkn> |
| Former Common name | R26Fucci2aR(RM185R), B6-ROSA26/Fucci2aR KI |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Richard Mort, MRC IGMM, University of Edinburgh in 2014. The mutant mice were corssed to C57BL/6. C57BL/6 mixed background. |
| Strain description | Fucci2aR ROSA26 knock-in mice. Targeted insertion of CAG-loxP-Neo-SV40 pA-loxP-Fucci2aR-bovine GH pA cassette into the mouse Rosa26 locus. |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6J] |
| References | Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Mort R L, Ford M J, Sakaue-Sawano A, Lindstrom N O, Casadio A, Douglas A T, Keighren M A, Hohenstein P, Miyawaki A, Jackson I J Cell Cycle, 13, 2681-2696 (2014). 25486356 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2025/04/21Room:3-CRack:KSentinel mouse program 2025/01/20Room:3-CRack:KSentinel mouse program 2024/11/13Room:3-CRack:KAdditional tests 2024/10/21Room:3-CRack:KSentinel mouse program 2024/07/22Room:3-CRack:KSentinel mouse program 2024/04/22Room:3-CRack:KSentinel mouse program 2024/01/22Room:3-CRack:KSentinel mouse program 2023/10/23Room:3-CRack:KSentinel mouse program 2023/07/24Room:3-CRack:KSentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| CDT1 | chromatin licensing and DNA replication factor (human) | 6 | CDT1 | ||||
| GH | Growth hormone polyA (Bovine) | 6 | GH | ||||
| GMNN | geminin (human) | 6 | GMNN | ||||
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<tm1(Fucci2aR)Jkn> | targeted mutation 1, Ian Jackson | |||
| T2A | Thosea asigna virus 2A peptide (insect) | 6 | T2A | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| mCherry | Red Fluorescent Protein (Discosoma sp.) | 6 | mCherry | ||||
| mVenus | Yellow Fluorescent Protein (Aequorea victoria) | 6 | mVenus | ||||
| neo | neomycin resistance gene (E. coli) | 6 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | ||||
| SV40 polyA | 6 | ||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), P1 phage loxP sites, E. coli neo, SV40 polyA, human CDT1 cDNA, Discosoma sp. mCherry cDNA, Thosea asigna virus 2A peptide (T2A), Jellyfish variant of enhanced yellow fluorescent protein (mVenus) cDNA, human GMNN cDNA, bovine GH polyA, mouse Rosa26 genomic DNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature(s) designated by the DEPOSITOR is requested. Cell Cycle 13:17, 2681-2696, 2014. 3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 5) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
| Depositor | Richard Mort (The University of Edinburgh) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |