Strain Data Sheet

RBRC06478

Strain Information

Image
BRC No.RBRC06478
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Fer1l4<tm1Osb>/5A
Former Common nameFer1l4#5A
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Miyata and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2013. EGR-G101 ES cells derived from a Tg mouse in C57BL/6N background were used. C57BL/6N background.
Strain descriptionFer1l4 knockout mice. Exon 5 and 6 were deleted.
Colony maintenance
References
Testis-enriched ferlin, FER1L5, is required for Ca2+-activated acrosome reaction and male fertility.
Akane Morohoshi, Haruhiko Miyata, Keizo Tokuhiro, Rie Iida-Norita, Taichi Noda, Yoshitaka Fujihara, Masahito Ikawa
Sci. Adv., 9(4):eade7607 (2023). 36696506

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • reproductive system phenotype(MP:0005389)
    • Detailed phenotype data

    Ordering Information

    Donor DNAchicken beta-actin promoter, rabbit beta-globin polyadenylation signal, CMV CMV IE enhancer, mouse proacrosin signal peptide, mouse acrosin N-ternimal peptide, jellyfish GFP cDNA, mouse acrosin promoter, mouse Fer1l4 genomic DNA, SV40 polyadenylation site, mouse PGK promoter, E.coli. Neomysin phosphotransferase gene (neoR), Yeast FRT site, Phage P1loxP site
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Adv. Jan 25;9(4):eade7607.(2023) doi: 10.1126/sciadv.ade7607. Epub 2023 Jan 25.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.　The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data