RBRC06449

Information1

Image
BRC No.RBRC06449
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6-H3t<em1Osb>
Former Common nameH3mmT KO, B6-H3mmT EN, H3mmT knockout mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Jun Ueda and Kazuo Yamagata, Research Institute for Microbial Diseases, Osaka University and Kazumitsu Maehara, Akihito Harada and Yasuyuki Ohkawa, Graduate School of Medical Sciences, Kyushu University in 2013. Generated by injecting Cas9 and gRNA (GCAAGGGAGGCGGACGATTCAGG) expression plasmids into the C57BL/6 fertilized eggs. Off-target analysis was not examined.
Strain descriptionSperm specific histone H3 variant, H3mmT gene knockout mice. A 16 bp including ATG was deleted. Homozygous mutant males are infertile.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
ReferencesCell Rep., 18(3):593-600 (2017). 28099840

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Trim17tripartite motif-containing 1711Trim17<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Information2

Donor DNA[hCas9 (addgene ID41815; CMV IE promoter, SV40 nls, human codon optimized Cas9*, neo*, TK pA), gRNA Cloning vector (addgene ID41824; human U6 polymerase III promoter, neo)] * Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep. 2017 Jan 17;18(3):593-600. doi: 10.1016/j.celrep.2016.12.065. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorKazuo Yamagata (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Category I MTA: CRISPR/Cas9 genome edited bioresources ( Japanese / English )

BRC mice in Publications

No Data