Strain Data Sheet

RBRC06396

Strain Information

Image
BRC No.RBRC06396
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Fez1<tm1Kei>
Former Common nameFez1 KO, B6-Fez1 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J (N7 generations).
Strain descriptionFez1 gene knockout mice. Exon 2 was replaced with a PGK-neo (loxP-neo) cassette.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Mice lacking the schizophrenia-associated protein FEZ1 manifest hyperactivity and enhanced responsiveness to psychostimulants.
Sakae N, Yamasaki N, Kitaichi K, Fukuda T, Yamada M, Yoshikawa H, Hiranita T, Tatsumi Y, Kira J, Yamamoto T, Miyakawa T, Nakayama K I
Human Molecular Genetics, 17, 3191-3203 (2008). 18647754

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signal9
Fez1fasciculation and elongation protein zeta 19Fez1targeted mutation 1, Keiichi I Nakayama
loxPphage P1 loxP9loxP
loxPphage P1 loxP9loxP
neoneomycin resistance gene (E. coli)9mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal associative learning(MP:0002062)

  • abnormal contextual conditioning behavior(MP:0001469)

  • abnormal cued conditioning behavior(MP:0001454)

  • behavior/neurological phenotype(MP:0005386)

  • decreased body weight(MP:0001262)
    • more 5 phenotypes
  • hyperactivity(MP:0001399)

  • increased dopamine level(MP:0001906)

  • increased stereotypic behavior(MP:0001409)

  • increased vertical activity(MP:0002574)

  • nervous system phenotype(MP:0003631)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, mouse phosphoglycerate kinase promoter, SV40 polyA signal, phage P1 loxP
    Research applicationCre/loxP system
    Neurobiology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorKeiichi Nakayama (Kyushu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP

    BRC mice in Publications

    No Data