Strain Data Sheet

RBRC06369

Information1

Image
BRC No.RBRC06369
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Fam135b<em1Rbrc>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Fam135bfamily with sequence similarity 135, member B15Fam135b<em1Rbrc>endonuclease mediated mutation 1, RIKEN BRC

Information2

Donor DNA(All genes were introduced in the form of RNA)Streptococcus pyogenes tracrRNA, crRNA (in the form of sgRNA) and Cas9, Synthetic DNA Flag tag, His tag, SV40 nuclear localization signal (NLS), Simian virus 5 V5 epitope tag, Bovine growth hormone (bGH) polyA signal
Research application
Specific Term and ConditionsThe RECIPIENT should acknowledge RIKEN BRC and the International Mouse Phenotyping Consortium for creating and providing of the knockout mice.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data