Strain Data Sheet

RBRC06357

Strain Information

Image
BRC No.RBRC06357
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Msr1<tm1(HBEGF)Mtka>
Former Common nameCD204-DTR, B6-Msr1/DTR KI
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Masato Tanaka, RIKEN Research Center for Allergy and Immunology in 2011. B6JN/1 ES cells were used. C57BL/6 background.
Strain descriptionCD204 (Msr1) DTR knock-in mice. HB-EGF is expressed from CD204 locus.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesBiochem Biophys Rep. Aug 7;23:100791 (2020). doi: 10.1016/j.bbrep.2020.100791. eCollection 2020 Sep. 32793817

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Msr1macrophage scavenger receptor 18Msr1<tm1(HBEGF)Mtka>targeted mutation 1, Masato TanakaCD204

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA8HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBEGFheparin-binding EGF-like growth factor (human)8HBEGF

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
gb2phage T7 gb2 promoter(synthetic)8gb2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAhuman HB-EGF gene cDNA, Rabbit beta-globin polyA, Saccharomyces cerevisiae FRT sites, mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli Neomycin resistance gene, mouse Msr1 genomic DNA
Research applicationFLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem Biophys Rep. 2020 Aug 7;23:100791. doi: 10.1016/j.bbrep.2020.100791. eCollection 2020 Sep.The recipient shall not distribute the biological resource to others without a written consent from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER (Masato Tanaka and Gen Nishitai), and then include them as co-authors in any publications resulted by the use of the BIOLOGICAL RESOURCE.
DepositorMasato Tanaka (Tokyo University of Pharmacy and Life Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data