Strain Data Sheet

RBRC06343

Strain Information

Image
BRC No.RBRC06343
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-App<tm2(NL-F)Tcs>
Former Common nameAPP<NL-F>, B6-App/NL-F KI
H-2 Haplotype
ES Cell lineiTL IC1 [C57BL/6NTac]
Background strain
Appearance
Strain developmentDeveloped by Takashi Saito and Takaomi C. Saido, RIKEN Brain Science Institute in 2009. C57BL/6 derived ES cells were used to generate the mutant mice. A floxed neo cassette was removed by crossing with EIIa-cre mice (B6.FVB-Tg(EIIa-cre)C5379Lmgd/J). C57BL/6 background.
Strain descriptionApp knock-in mice having Swedish mutation (NL) (RBRC06342), Swedish/Iberian (NL-F) mutations (RBRC06343) and Swedish/Iberian/Arctic mutations (NL-G-F) (RBRC06344). Homozygous mutant mice are viable and fertile.
Colony maintenanceHomozygote ×Homozygote [or Crossing to C57BL/6J(Crl)]
References
Single App knock-in mouse models of Alzheimer's disease.
Takashi Saito, Yukio Matsuba, Naomi Mihira, Jiro Takano, Per Nilsson, Shigeyoshi Itohara, Nobuhisa Iwata, Takaomi C Saido
Nat. Neurosci., 17(5):661-663 (2014). 24728269

Recent Advances in the Modeling of Alzheimer's Disease.
Hiroki Sasaguri, Shoko Hashimoto, Naoto Watamura, Kaori Sato, Risa Takamura, Kenichi Nagata, Satoshi Tsubuki, Toshio Ohshima, Atsushi Yoshiki, Kenya Sato, Wakako Kumita, Erika Sasaki, Shinobu Kitazume, Per Nilsson, Bengt Winblad, Takashi Saito, Nobuhisa Iwata, Takaomi C Saido
Front. Neurosci., 16:807473 (2022). 35431779

Human MAPT knockin mouse models of frontotemporal dementia for the neurodegenerative research community.
Takahiro Morito, Mohan Qi, Naoko Kamano, Hiroki Sasaguri, Sumi Bez, Martha Foiani, Karen Duff, Seico Benner, Toshihiro Endo, Hiroshi Hama, Hiroshi Kurokawa, Atushi Miyawaki, Hiroshi Mizuma, Naruhiko Sahara, Masafumi Shimojo, Makoto Higuchi, Takaomi C Saido, Naoto Watamura
Cell Rep. Methods, 5(4):101024 (2025). 40220760

Health Report

Examination Date / Room / Rack2026/01/05Room:4-BRack:CSentinel mouse program
2025/10/20Room:4-BRack:CSentinel mouse program
2025/08/04Room:4-BRack:CSentinel mouse program
2025/05/26Room:4-BRack:CSentinel mouse program
2025/02/25Room:4-BRack:CSentinel mouse program
2025/01/09Room:4-BRack:CAdditional tests
2024/11/25Room:4-BRack:CSentinel mouse program
2024/08/26Room:4-BRack:CSentinel mouse program
2024/05/27Room:4-BRack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
App
MGI:88059		amyloid beta precursor protein16App<tm2(NL-F)Tcs>
MGI:5637816		targeted mutation 2, Takaomi C. Saido
  • Alzheimer disease type 1(MedGEN)

  • Alzheimer disease(DisGeNET)

  • cerebral amyloid angiopathy, APP-related(MedGEN)
    • Frt yeast FRT (flippase recombination target) site16Frt
    loxP phage P1 loxP16loxP

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal learning/memory/conditioning (MP:0002063)

  • abnormal synapse morphology (MP:0009538)

  • amyloid beta deposits (MP:0003329)

  • amyloidosis (MP:0000604)

  • astrocytosis (MP:0003354)
    • more 1 phenotypes
  • microgliosis (MP:0008918)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP site, yeast FRT (flipase recombination target) site, mouse App genomic DNA (mutant type)
    Research applicationCre/loxP system
    FLP/frt system
    Mouse Models for Human Disease
    Specific Term and ConditionsThe BIOLOGICAL RESOURCE is the property of the RIKEN (The Institute of Physical and Chemical Research). RECIPIENT agrees to acknowledge Takashi SAITO and Takaomi C. SAIDO in an acknowledgement section. RECIPIENT also agrees to cite Nature Neuroscience 17, 661-663 (2014) in all publications obtained using BIOLOGICAL RESOURCE. RECIPIENT agrees to acknowledge DEPOSITOR in all oral presentations and written publications as related to the BIOLOGICAL RESOURCE. RECIPIENT shall keep confidential any or all information marked CONFIDENTIAL that is received from DEPOSITOR and related to the BIOLOGICAL RESOURCE including oral discussions if they are reduced to writing within thirty (30) days and are marked "CONFIDENTIAL". For-profit organizations must contact the DEPOSITOR (takaomi.saido@riken.jp) for a licensing contract. If any invention is conceived and reduced to practice by RECIPIENT in the performance of the Research Plan involving the BIOLOGICAL RESOURCE during the term of this MTA, RECIPIENT agrees to promptly inform the invention to DEPOSITOR and the parties will consult each other to determine inventorship and ownership of the invention based on the respective parties contribution to the invention, before filing an application for a patent. In the case of a jointly owned application, the parties will separately enter into a joint application agreement including the sharing of ownership, patent costs and licensing income, as well as the responsible party for the application procedures etc.
    DepositorTakaomi C. Saido (RIKEN CBS)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (RIKEN Bio Co., Ltd.
    Genotyping protocol -PCR-
    Mouse of the Month Sep 2014

    BRC mice in Publications

    McFadden SA, Fang Y, Quinn K, Peck MR, Chapman JE, Hill T, Bartke A, Hascup ER, Hascup KN.
    Surgical removal of visceral adipose tissue has therapeutic benefit in male APPNL-F mice.
    Front Endocrinol (Lausanne) 17 1681801(2026) 41847455
    Bellio TA, Krunic A, Campion MS, Dupaguntla R, Labadorf A, Stein TD, Lin H, Mellott TJ, Blusztajn JK.
    Perinatal Choline Supplementation Promotes Resilience Against Progression of Alzheimer's Disease-Like Brain Transcriptomic Signatures in AppNL-G-F Mice.
    Aging Cell e70148(2025) 41081549
    Abi-Ghanem C, Opiela AK, Paul AS, Comito ML, Hao L, Martino G, Kyaw NR, Salinero AE, Mansour FM, Kelly RD, Mutahi AM, Sura A, Thrasher CA, Groom EA, Batchelder MR, Zuloaga KL.
    Loss of ovarian hormones is detrimental in early disease stages of mouse models of Alzheimer's disease and multi-etiology dementia.
    Biol Sex Differ 17(1) 4(2025) 41345983
    Fang Y, Peck MR, Quinn K, Chapman JE, Medina D, McFadden SA, Bartke A, Hascup ER, Hascup KN.
    Senolytic intervention improves cognition, metabolism, and adiposity in female APPNL-F/NL-F mice.
    Geroscience 47(1) 1123-1138(2025) 39120687
    Findley CA, McFadden SA, Hill T, Peck MR, Quinn K, Hascup KN, Hascup ER.
    Sexual dimorphism, altered hippocampal glutamatergic neurotransmission, and cognitive impairment in APP knock-in mice.
    J Alzheimers Dis 102(2) 491-505(2024) 39543985
    Abi-Ghanem C, Salinero AE, Smith RM, Kelly RD, Belanger KM, Richard RN, Paul AS, Herzog AA, Thrasher CA, Rybka KA, Riccio D, Gannon OJ, Kordit D, Kyaw NR, Mansour FM, Groom E, Brooks HL, Robison LS, Pumiglia K, Zuloaga DG, Zuloaga KL.
    Effects of Menopause and High Fat Diet on Metabolic Outcomes in a Mouse Model of Alzheimer's Disease.
    J Alzheimers Dis 101(4) 1177-1194(2024) 39302361
    Honda K, Takahashi H, Hata S, Abe R, Saito T, Saido TC, Taru H, Sobu Y, Ando K, Yamamoto T, Suzuki T.
    Suppression of the amyloidogenic metabolism of APP and the accumulation of Aβ by alcadein α in the brain during aging.
    Sci Rep 14(1) 18471(2024) 39122814
    Hata S, Saito H, Kakiuchi T, Fukumoto D, Yamamoto S, Kasuga K, Kimura A, Moteki K, Abe R, Adachi S, Kinoshita S, Yoshizawa-Kumagaye K, Nishio H, Saito T, Saido TC, Yamamoto T, Nishimura M, Taru H, Sobu Y, Ohba H, Nishiyama S, Harada N, Ikeuchi T, Tsukada H, Ouchi Y, Suzuki T.
    Brain p3-Alcβ peptide restores neuronal viability impaired by Alzheimer's amyloid β-peptide.
    EMBO Mol Med 15(5) e17052(2023) 36994913
    Findley CA, McFadden SA, Cox MF, Sime LN, Peck MR, Quinn K, Bartke A, Hascup KN, Hascup ER.
    Prodromal Glutamatergic Modulation with Riluzole Impacts Glucose Homeostasis and Spatial Cognition in Alzheimer's Disease Mice.
    J Alzheimers Dis 94(1) 371-392(2023) 37248899
    Mowry FE, Espejo-Porras F, Jin S, Quadri Z, Wu L, Bertolio M, Jarvis R, Reynolds C, Alananzeh R, Bieberich E, Yang Y.
    Chronic nSMase inhibition suppresses neuronal exosome spreading and sex-specifically attenuates amyloid pathology in APP knock-in Alzheimer's disease mice.
    Neurobiol Dis 184 106213(2023) 37364689
    Shang Y, Sun X, Chen X, Wang Q, Wang EJ, Miller E, Xu R, Pieper AA, Qi X.
    A CHCHD6-APP axis connects amyloid and mitochondrial pathology in Alzheimer's disease.
    Acta Neuropathol 144(5) 911-938(2022) 36104602
    Hiroki Sasaguri, Shoko Hashimoto, Naoto Watamura, Kaori Sato, Risa Takamura, Kenichi Nagata, Satoshi Tsubuki, Toshio Ohshima, Atsushi Yoshiki, Kenya Sato, Wakako Kumita, Erika Sasaki, Shinobu Kitazume, Per Nilsson, Bengt Winblad, Takashi Saito, Nobuhisa Iwata, Takaomi C. Saido
    Recent Advances in the Modeling of Alzheimer’s Disease
    Frontiers Neuroscience 16 (2022) 35431779
    Hata S, Kano K, Kikuchi K, Kinoshita S, Sobu Y, Saito H, Saito T, Saido TC, Sano Y, Taru H, Aoki J, Komano H, Tomita T, Natori S, Suzuki T.
    Suppression of amyloid-β secretion from neurons by cis-9, trans-11-octadecadienoic acid, an isomer of conjugated linoleic acid.
    J Neurochem 159(3) 603-617(2021) 34379812
    Sauerbeck AD, Gangolli M, Reitz SJ, Salyards MH, Kim SH, Hemingway C, Gratuze M, Makkapati T, Kerschensteiner M, Holtzman DM, Brody DL, Kummer TT.
    SEQUIN Multiscale Imaging of Mammalian Central Synapses Reveals Loss of Synaptic Connectivity Resulting from Diffuse Traumatic Brain Injury.
    Neuron 107(2) 257-273.e5(2020) 32392471