Strain Information | |
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Image | |
BRC No. | RBRC06343 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-App<tm2(NL-F)Tcs> |
Former Common name | APP<NL-F>, B6-App/NL-F KI |
H-2 Haplotype | |
ES Cell line | iTL IC1 [C57BL/6NTac] |
Background strain | |
Appearance | |
Strain development | Developed by Takashi Saito and Takaomi C. Saido, RIKEN Brain Science Institute in 2009. C57BL/6 derived ES cells were used to generate the mutant mice. A floxed neo cassette was removed by crossing with EIIa-cre mice (B6.FVB-Tg(EIIa-cre)C5379Lmgd/J). C57BL/6 background. |
Strain description | App knock-in mice having Swedish mutation (NL) (RBRC06342), Swedish/Iberian (NL-F) mutations (RBRC06343) and Swedish/Iberian/Arctic mutations (NL-G-F) (RBRC06344). Homozygous mutant mice are viable and fertile. |
Colony maintenance | Homozygote ×Homozygote [or Crossing to C57BL/6J(Crl)] |
References | Single App knock-in mouse models of Alzheimer's disease. Saito T, Matsuba Y, Mihira N, Takano J, Nilsson P, Itohara S, Iwata N, Saido T C Nat. Neurosci., 17(5):661-663 (2014). 24728269Recent Advances in the Modeling of Alzheimer's Disease. Hiroki Sasaguri, Shoko Hashimoto, Naoto Watamura, Kaori Sato, Risa Takamura, Kenichi Nagata, Satoshi Tsubuki, Toshio Ohshima, Atsushi Yoshiki, Kenya Sato, Wakako Kumita, Erika Sasaki, Shinobu Kitazume, Per Nilsson, Bengt Winblad, Takashi Saito, Nobuhisa Iwata, Takaomi C Saido Front. Neurosci., 16:807473 (2022). 35431779 |
Health Report | |
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Examination Date / Room / Rack | 2024/08/26Room:4-BRack:C 2024/05/27Room:4-BRack:C 2024/02/26Room:4-BRack:C 2023/12/04Room:4-BRack:C 2023/11/27Room:4-BRack:C 2023/08/28Room:4-BRack:C 2023/05/29Room:4-BRack:C 2023/02/27Room:4-BRack:C 2023/02/13Room:4-BRack:C |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
App | amyloid beta precursor protein | 16 | App | targeted mutation 2, Takaomi C. Saido | |||
Frt | yeast FRT (flippase recombination target) site | 16 | Frt | ||||
loxP | phage P1 loxP | 16 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 1 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | phage P1 loxP site, yeast FRT (flipase recombination target) site, mouse App genomic DNA (mutant type) |
Research application | Cre/loxP system FLP/frt system Mouse Models for Human Disease |
Specific Term and Conditions | The BIOLOGICAL RESOURCE is the property of the RIKEN (The Institute of Physical and Chemical Research). RECIPIENT agrees to acknowledge Takashi SAITO and Takaomi C. SAIDO in an acknowledgement section. RECIPIENT also agrees to cite Nature Neuroscience 17, 661-663 (2014) in all publications obtained using BIOLOGICAL RESOURCE. RECIPIENT agrees to acknowledge DEPOSITOR in all oral presentations and written publications as related to the BIOLOGICAL RESOURCE. RECIPIENT shall keep confidential any or all information marked CONFIDENTIAL that is received from DEPOSITOR and related to the BIOLOGICAL RESOURCE including oral discussions if they are reduced to writing within thirty (30) days and are marked "CONFIDENTIAL". For-profit organizations must contact the DEPOSITOR (takaomi.saido@riken.jp) for a licensing contract. If any invention is conceived and reduced to practice by RECIPIENT in the performance of the Research Plan involving the BIOLOGICAL RESOURCE during the term of this MTA, RECIPIENT agrees to promptly inform the invention to DEPOSITOR and the parties will consult each other to determine inventorship and ownership of the invention based on the respective parties contribution to the invention, before filing an application for a patent. In the case of a jointly owned application, the parties will separately enter into a joint application agreement including the sharing of ownership, patent costs and licensing income, as well as the responsible party for the application procedures etc. |
Depositor | Takaomi C. Saido (RIKEN CBS) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Sep 2014 |
BRC mice in Publications |
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Hiroki Sasaguri, Shoko Hashimoto, Naoto Watamura, Kaori Sato, Risa Takamura, Kenichi Nagata, Satoshi Tsubuki, Toshio Ohshima, Atsushi Yoshiki, Kenya Sato, Wakako Kumita, Erika Sasaki, Shinobu Kitazume, Per Nilsson, Bengt Winblad, Takashi Saito, Nobuhisa Iwata, Takaomi C. Saido Recent Advances in the Modeling of Alzheimer’s Disease Frontiers Neuroscience 16 (2022) 35431779 |
Hata S, Kano K, Kikuchi K, Kinoshita S, Sobu Y, Saito H, Saito T, Saido TC, Sano Y, Taru H, Aoki J, Komano H, Tomita T, Natori S, Suzuki T. Suppression of amyloid-β secretion from neurons by cis-9, trans-11-octadecadienoic acid, an isomer of conjugated linoleic acid. J Neurochem 159(3) 603-617(2021) 34379812 |
Mowry FE, Espejo-Porras F, Jin S, Quadri Z, Wu L, Bertolio M, Jarvis R, Reynolds C, Alananzeh R, Bieberich E, Yang Y. Chronic nSMase inhibition suppresses neuronal exosome spreading and sex-specifically attenuates amyloid pathology in APP knock-in Alzheimer's disease mice. Neurobiol Dis 184 106213(2023) 37364689 |
Sauerbeck AD, Gangolli M, Reitz SJ, Salyards MH, Kim SH, Hemingway C, Gratuze M, Makkapati T, Kerschensteiner M, Holtzman DM, Brody DL, Kummer TT. SEQUIN Multiscale Imaging of Mammalian Central Synapses Reveals Loss of Synaptic Connectivity Resulting from Diffuse Traumatic Brain Injury. Neuron 107(2) 257-273.e5(2020) 32392471 |
Honda K, Takahashi H, Hata S, Abe R, Saito T, Saido TC, Taru H, Sobu Y, Ando K, Yamamoto T, Suzuki T. Suppression of the amyloidogenic metabolism of APP and the accumulation of Aβ by alcadein α in the brain during aging. Sci Rep 14(1) 18471(2024) 39122814 |