Strain Data Sheet

RBRC06336

Strain Information

Image
BRC No.RBRC06336
TypeTargeted Mutation and Spontaneous Mutation CongenicCartagena
SpeciesMus musculus
Strain nameNOD.Cg-Prkdc<scid> E2f1<tm1Meg>
Former Common nameNOD.e2f1<+/->, NOD-scid/E2f1 KO
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Hidenobu Senpuku, National Institute of Infectious Diseases. The E2f1<tm1Meg> mice of C57BL/6 and 129 mixed background were backcrossed to NOD/LtJ mice, and furthermore the mutant mice were backcrossed to NOD/SCID mice.
Strain descriptionE2f1 deficient NOD/SCID mice. Part of exon 3 to exon 4 of E2f1 gene was replaced with a neo cassette. E2f1 homozygous mutant mice are immunodeficiency and show decreased saliva production.
Colony maintenanceE2f1: Heterozygote x Wild-type; scid: Homozygote x Homozygote [or Crossing to NOD/ShiJic-scid/Jcl]
References
E2F-1 functions in mice to promote apoptosis and suppress proliferation.
S J Field, F Y Tsai, F Kuo, A M Zubiaga, W G Kaelin, D M Livingston, S H Orkin, M E Greenberg
Cell, 85, 549-561 (1996). 8653790

E2f1 mutation induces early onset of diabetes and Sjögren's syndrome in nonobese diabetic mice.
Mohammad Abdus Salam, Khairul Matin, Naoko Matsumoto, Yuzo Tsuha, Nobuhiro Hanada, Hidenobu Senpuku
J. Immunol., 173, 4908-4918 (2004). 15470032

E2F-1-deficient NOD/SCID mice developed showing decreased saliva production.
Hikaru Matsui-Inohara, Hiroshi Uematsu, Takanori Narita, Keitaro Satoh, Hideo Yonezawa, Koichiro Kuroda, Tatsuro Ito, Saori Yoneda, Taketo Kawarai, Hiroshi Sugiya, Haruo Watanabe, Hidenobu Senpuku
Exp. Biol. Med. (Maywood)., 234, 1525-1536 (2009). 19934373

Roles of salivary components in Streptococcus mutans colonization in a new animal model using NOD/SCID.e2f1-/- mice.
Tatsuro Ito, Takahide Maeda, Hidenobu Senpuku
PLoS One. 7: e32063 (2012). 22363797

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Prkdc
MGI:104779		protein kinase, DNA activated, catalytic polypeptide16Prkdc<scid>
MGI:1857113		severe combined immunodeficiency
  • severe combined immunodeficiency due to DNA-PKcs deficiency(MedGEN)
    • E2f1
    MGI:101941    		E2F transcription factor 12E2f1<tm1Meg>
    MGI:1857424    		targeted mutation 1, Michael E Greenburg
    neo neomycin resistance gene (E. coli)2 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal CD4-positive, alpha beta T cell morphology (MP:0002432)

  • abnormal CD8-positive, alpha beta T cell morphology (MP:0005010)

  • abnormal NK cell physiology (MP:0010766)

  • abnormal Peyer's patch morphology (MP:0000696)

  • abnormal cell cycle (MP:0003077)
    • more 81 phenotypes
  • abnormal cell death (MP:0000313)

  • abnormal complement pathway (MP:0002471)

  • abnormal cone electrophysiology (MP:0004022)

  • abnormal double-positive T cell morphology (MP:0002408)

  • abnormal humoral immune response (MP:0001800)

  • abnormal intestinal epithelium morphology (MP:0000488)

  • abnormal level of surface class II molecules (MP:0005042)

  • abnormal lymph node B cell domain morphology (MP:0002344)

  • abnormal lymph node morphology (MP:0002339)

  • abnormal pancreatic islet morphology (MP:0005215)

  • abnormal response to transplant (MP:0005671)

  • abnormal retina bipolar cell morphology (MP:0006073)

  • abnormal retina progenitor cell morphology (MP:0006071)

  • abnormal spleen white pulp morphology (MP:0002357)

  • abnormal thymus corticomedullary boundary morphology (MP:0009543)

  • abnormal thymus lobule morphology (MP:0002367)

  • abnormal thymus morphology (MP:0000703)

  • absent B cells (MP:0008071)

  • absent Peyer's patches (MP:0002831)

  • absent pre-B cells (MP:0000238)

  • absent thymus cortex (MP:0010250)

  • decreased B cell number (MP:0005017)

  • decreased B cell proliferation (MP:0005093)

  • decreased CD4-positive, alpha-beta T cell number (MP:0008075)

  • decreased CD8-positive, alpha-beta T cell number (MP:0008079)

  • decreased IgG level (MP:0001805)

  • decreased NK cell number (MP:0008045)

  • decreased T cell number (MP:0005018)

  • decreased T cell proliferation (MP:0005095)

  • decreased apoptosis (MP:0006043)

  • decreased body weight (MP:0001262)

  • decreased bone marrow cell number (MP:0000333)

  • decreased circulating insulin level (MP:0002727)

  • decreased double-positive T cell number (MP:0005092)

  • decreased erythrocyte cell number (MP:0002875)

  • decreased immunoglobulin level (MP:0002460)

  • decreased leukocyte cell number (MP:0000221)

  • decreased level of surface class I molecules (MP:0001841)

  • decreased lymphocyte cell number (MP:0005016)

  • decreased mature B cell number (MP:0008211)

  • decreased pancreatic beta cell number (MP:0003339)

  • decreased pre-B cell number (MP:0008209)

  • decreased regulatory T cell number (MP:0004974)

  • decreased retina ganglion cell number (MP:0006309)

  • decreased salivation (MP:0000623)

  • enlarged thymus (MP:0000709)

  • hyperglycemia (MP:0001559)

  • impaired complement classical pathway (MP:0002473)

  • impaired natural killer cell mediated cytotoxicity (MP:0005070)

  • increased CD4-positive, alpha-beta T cell number (MP:0008074)

  • increased CD8-positive, alpha-beta T cell number (MP:0008078)

  • increased NK cell number (MP:0008044)

  • increased T cell derived lymphoma incidence (MP:0002024)

  • increased T cell number (MP:0005015)

  • increased cellular sensitivity to X-ray irradiation (MP:0002879)

  • increased circulating glucose level (MP:0005559)

  • increased eosinophil cell number (MP:0005011)

  • increased granulocyte number (MP:0000322)

  • increased interferon-gamma secretion (MP:0008566)

  • increased interleukin-4 secretion (MP:0008699)

  • increased length of allograft survival (MP:0004751)

  • increased macrophage cell number (MP:0005425)

  • increased mean corpuscular volume (MP:0002590)

  • increased monocyte cell number (MP:0000220)

  • increased neutrophil cell number (MP:0000219)

  • increased susceptibility to autoimmune diabetes (MP:0004803)

  • increased thymocyte number (MP:0000714)

  • increased thymus weight (MP:0004955)

  • insulitis (MP:0004031)

  • lymph node hypoplasia (MP:0008101)

  • lymphoid hypoplasia (MP:0002223)

  • premature death (MP:0002083)

  • salivary gland inflammation (MP:0001870)

  • small Peyer's patches (MP:0008135)

  • small lymphoid organs (MP:0000687)

  • small thymus (MP:0000706)

  • spleen hypoplasia (MP:0000694)

  • stomach inflammation (MP:0001873)

  • testicular atrophy (MP:0003205)

  • thin retina outer nuclear layer (MP:0008515)

  • thymus cyst (MP:0014130)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse E2f1 genomic DNA
    Research applicationImmunology and Inflammation Research
    Mouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Biol. Med. (Maywood)., 234, 1525-1536 (2009).
    DepositorHidenori Senpuku (National Institute of Infectious Diseases)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data