Strain Data Sheet

RBRC06331

Strain Information

Image
BRC No.RBRC06331
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6;129-Dtx4<Gt(431E3)Cmhd>
Former Common nameDtx4 KO, B6-Dtx4 Gt
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentMassachusetts General Hospital・Naifang Lu、Athanasia Spandidos、Brian Seed。Canadian Mouse Mutant RepositoryのジーントラップESクローン（CMHD-GT_431-E3-3）を用いて作出。C57BL/6と129の混合背景。
Strain descriptionDtx4遺伝子のジーントラップマウス (CMMR、Vector: pNMDi4、CMHD-GT_431-E3-3クローン) 。ホモマウスは外見上正常。詳細は未解析。
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NTac]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dtx4deltex 4 homolog (Drosophila)19Dtx4<Gt(431E3)Cmhd>gene trap 431E3, Centre for Modeling Human Disease

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)19GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hprtmouse Hprt splice donor (SD)19Hprt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SAhuman adenovirus type 3/5 SA (splice acceptor)19SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)19Venus

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)19mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

供与核酸Human adenovirus type 3/5 SA (splice acceptor), flexible linker (FL), jellyfish Venus, P1 phage loxP, Eukaryotic translation initiation factor 3 subunit 7, BGH polyA, mouse PGK promoter, E. coli neomycin resistance gene, mouse Hprt SD (splice donor)
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって謝辞の表明を必要とする。
研究成果の公表にあたってVenusの開発者である宮脇敦史先生への謝辞の表明並びに指定する文献を引用する。Nat. Biotechnol., 20, 87-90 (2002).
DepositorBrian Seed（Massachusetts General Hospital）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data