Strain Data Sheet

RBRC06325

Strain Information

Image
BRC No.RBRC06325
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nfatc2<tm1Rao> Tob1<tm1Tya>
Former Common nameNFAT-c2/Tob-1 double KO, DKO, B6-Nfatc2/Tob1 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentUniversity of Minnesota, Jamie Modiano. Nfatc2 KO（Harvard Medical School, Anjana Rao）とTob1 KO（東京大学医科学研究所 山本　雅 先生）マウスとの交配により作出。
Strain descriptionNfatc2遺伝子とTob1遺伝子のダブルノックアウトマウス。Nfatc2遺伝子のN末端エクソンをPGK-neoカセットで置換、Tob1遺伝子はエクソン (1エクソンからなる) をPGK-neoカセットで置換。6ヶ月齢のダブルホモマウスは、外見上特に目立った異常は認められない (Nfatc2シングルホモマウスは15-18ヶ月齢で約50%がB-cell lymphomasを発症する) 。Nfatc2ホモ/Tob1へテロ型の雌マウスは繁殖するもののミルクの分泌異常のため育仔が困難 (交配に使用する場合は里親を使用する) (ダブルホモ雌マウスは不妊) 。Nfatc2ホモ/Tob1ホモ型の雄マウスを交配に使用した場合は、産仔数が多くなり、異常分娩が多くなる。
Colony maintenanceNfatc2: Homozygote x Homozygote; Tob1: Wild-type (female) x Heterozygote (male) [or Crossing to C57BL/6J(Crlj)]
ReferencesCell, 103, 1085-1097 (2000). 11163184
PLoS One, 9, e100629 (2014). 24945807
Science, 272, 892-895 (1996). 8629027

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tob1transducer of ErbB-2.111Tob1<tm1Tya>targeted mutation 1, Tadashi Yamamoto

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nfatc2nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 22Nfatc2<tm1Rao>targeted mutation 1, Anjana Rao

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

供与核酸mouse Nfatc2 genomic DNA, mouse Tob1 genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene
Research application
提供条件条件を付加する。
1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLOS One, 9, e100629 (2014). 2) Recipient will not backcross the BIOLOGICAL RESOURCE to establish breeding colonies for either the NFAT1 KO mice or the Tob-1KO mice. 3) Tob-1 KO mice are the property of the Okinawa Institute of Science and Technology Graduate University and are available from the RIKEN BRC. The RECIPIENT must inform the DEVELOPER (Professor Tadashi Yamamoto, Okinawa Institute of Science and Technology Graduate University) the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from him. The RECIPIENT agrees to use the BIOLOGICAL RESOUCE as collaboration with the DEVELOPER in case his ongoing research is overlapping with that of the RECIPIENT. 4) NFAT1 KO mice are the property of the LaJolla Institute for Allergy and Immunology and will not be further distributed to others. Any requests for the NFAT1 KO mice shall be referred to the LaJolla Institute for Allergy and Immunology. 5) Recipient shall acquire prior written consent from the Depositor for use of the BIOLOGICAL RESOURCE by for-profit institutions or for commercial research purposes. 6) Recipient shall acquire prior written consent from the Depositor before filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
DepositorJaime F. Modiano（University of Minnesota）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data