Strain Data Sheet

RBRC06294

Strain Information

Image
BRC No.RBRC06294
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Aqp7<tm1Tfun>
Former Common nameAqp7 KO, B6-Aqp7 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Norikazu Maeda, Tohru Funahashi and Iichiro Shimomura. 129 derived ES cells were used. The mutant mice were crossed to C57BL/6 over 5 times.
Strain descriptionAquaporin 7 (Aqp7) gene knockout mice. Exon 1-3 containing the translation start site was replaced with a neo cassette.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
References
Adaptation to fasting by glycerol transport through aquaporin 7 in adipose tissue.
Maeda N, Funahashi T, Hibuse T, Nagasawa A, Kishida K, Kuriyama H, Nakamura T, Kihara S, Shimomura I, Matsuzawa Y
Proc. Natl. Acad. Sci. USA, 101, 17801-17806 (2004). 15591341

Aquaporin 7 deficiency is associated with development of obesity through activation of adipose glycerol kinase.
Hibuse T, Maeda N, Funahashi T, Yamamoto K, Nagasawa A, Mizunoya W, Kishida K, Inoue K, Kuriyama H, Nakamura T, Fushiki T, Kihara S, Shimomura I
Proc. Natl. Acad. Sci. USA, 102, 10993-10998 (2005). 16009937

The heart requires glycerol as an energy substrate through aquaporin 7, a glycerol facilitator.
Hibuse T, Maeda N, Nakatsuji H, Tochino Y, Fujita K, Kihara S, Funahashi T, Shimomura I
Cardiovasc. Res., 83, 34-41 (2009). 19297367

Health Report

Examination Date / Room / Rack2023/07/24Room:3-CRack:GSentinel mouse program
2023/04/24Room:3-CRack:GSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aqp7
MGI:1314647		aquaporin 74Aqp7<tm1Tfun>
MGI:3526441		targeted mutation 1, Tohru Funahashi
loxP phage P1 loxP4loxP
loxP phage P1 loxP4loxP
neo neomycin resistance gene (E. coli)4 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cardiac muscle contractility(MP:0002972)

  • decreased circulating glycerol level(MP:0003442)

  • hypoglycemia(MP:0000189)

  • increased glycerol level(MP:0003441)

  • increased myocardial infarct size(MP:0003037)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Pgk1 polyA, mouse Aqp7 genomic DNA
    Research applicationCardiovascular Research
    Cre/loxP system
    Metabolism Research
    covid19_progression
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Nat.l Acad. Sci. USA, 101, 17801-17806 (2004). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorIichiro Shimomura (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Fujii M, Bessho R, Akimoto T, Ishii Y.
    Cardioprotective effect of St. Thomas' Hospital No. 2 solution against age-related changes in aquaporin-7-deficient mice.
    Gen Thorac Cardiovasc Surg (2023) 37691043
    Ishihama S, Yoshida S, Yoshida T, Mori Y, Ouchi N, Eguchi S, Sakaguchi T, Tsuda T, Kato K, Shimizu Y, Ohashi K, Okumura T, Bando YK, Yagyu H, Wettschureck N, Kubota N, Offermanns S, Kadowaki T, Murohara T, Takefuji M.
    LPL/AQP7/GPD2 promotes glycerol metabolism under hypoxia and prevents cardiac dysfunction during ischemia.
    FASEB J 35(12) e22048(2021) 34807469
    Fujii M, Ota K, Bessho R.
    Cardioprotective effect of hyperkalemic cardioplegia in an aquaporin 7-deficient murine heart.
    Gen Thorac Cardiovasc Surg 68(6) 578-584(2020) 31707553
    Raje V, Ahern KW, Martinez BA, Howell NL, Oenarto V, Granade ME, Kim JW, Tundup S, Bottermann K, Gödecke A, Keller SR, Kadl A, Bland ML, Harris TE.
    Adipocyte lipolysis drives acute stress-induced insulin resistance.
    Sci Rep 10(1) 18166(2020) 33097799