Strain Information | |
---|---|
Image | |
BRC No. | RBRC06292 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Ngly1<tm1Tasuz> |
Former Common name | Ngly1 floxed, B6-Ngly1 floxed |
H-2 Haplotype | |
ES Cell line | KY1.1[(C57BL/6J x 129S6/SvEvTac)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Tadashi Suzuki, Global Research Cluster, RIKEN. KY1.1 ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. Neo cassette was removed by crossing with FLP mice (B6). The mutant mice were backcrosses to C57BL/6 over 11 times. |
Strain description | Ngly floxed mice. Exons 11 and 12 were flanked by loxP sites. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6J(Crj)] |
References | Biochim Biophys Acta Mol Basis Dis., 13:165588 (2019). 31733337 Proc. Natl. Acad. Sci. USA, 112 (5) 1398-403 (2015). 25605922 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site14Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP14loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP14loxP |
Ordering Information | |
---|---|
Donor DNA | mouse Mouse Ngly1 genomic DNA, Phage P1 loxP, yeast FRT |
Research application | Cre/loxP system FLP/frt system Mouse Models for Human Disease Neurobiology Research |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochim Biophys Acta Mol Basis Dis. 2019 Nov 13:165588 |
Depositor | Tadashi Suzuki (RIKEN) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |